Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, PR China.
Department of Neurosurgery, Nanjing Children's Hospital, Nanjing, PR China.
PLoS One. 2019 Apr 5;14(4):e0215069. doi: 10.1371/journal.pone.0215069. eCollection 2019.
To investigate the value of dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) for evaluating denervated skeletal muscle in rabbits.
24 male rabbits were randomly divided into an irreversible neurotmesis group and a control group. In the experimental group, the sciatic nerves of rabbits were transected for irreversible neurotmesis model. A sham operation was performed in the control group. MRI of rabbit lower legs was performed before nerve surgery and 1 day, 3 days, 5 days, 1 week, 2 weeks, 3 weeks, 4 weeks, 6 weeks, 8 weeks, 10 weeks, and 12 weeks after surgery.
Signal intensity changes were seen in the left gastrocnemius muscle on the T2-weighted images. DCE-MRI derived parameters (Ktrans, Kep, and Vp) were measured in vivo. In the irreversible neurotmesis group, T2-weighted images showed increased signal intensity in the left gastrocnemius muscle. Ktrans, Vp values changes occur as early as 1 day after denervation, and increased gradually until 4 weeks after surgery. There are significant increases in both Ktrans and Vp values compared with those in the control group after surgery (P < 0.05). Kep values show no significant difference between the irreversible neurotmesis group and the control group.
DCE-MRI hold the promise of an early and sensitive diagnosis of denervated skeletal muscle.
探讨动态对比增强磁共振成像（DCE-MRI）评价去神经化骨骼肌的价值。
24 只雄性兔随机分为不可逆神经切断组和对照组。实验组切断兔坐骨神经制作不可逆神经切断模型，对照组行假手术。术前及术后 1、3、5、1 周、2 周、3 周、4 周、6 周、8 周、10 周、12 周行兔下肢 MRI 检查。
T2 加权像上可见左侧比目鱼肌信号强度变化。DCE-MRI 衍生参数（Ktrans、Kep 和 Vp）进行活体测量。不可逆神经切断组术后 1 天即出现左侧比目鱼肌 T2 加权像信号强度增加，Ktrans、Vp 值变化早于术后 1 天，直至术后 4 周逐渐增加，术后均明显高于对照组（P < 0.05）。Kep 值在不可逆神经切断组与对照组之间无显著差异。
DCE-MRI 有望早期、敏感地诊断去神经化骨骼肌。
