利用低密度阵列验证吸烟的头颈部癌症患者和有癌前病变的个体/出租车司机外周血中候选基因的表达谱。

Validation of gene expression profiles of candidate genes using low density array in peripheral blood of tobacco consuming head and neck cancer patients and auto/taxi drivers with preneoplastic lesions.

机构信息

Developmental Toxicology Laboratory, System Toxicology & Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow 226001, U.P., India; Babu Banarsi Das University, Faizabad Road, Lucknow 226028, U.P., India.

出版信息

Genomics. 2020 Jan;112(1):513-519. doi: 10.1016/j.ygeno.2019.04.003. Epub 2019 Apr 2.

DOI:10.1016/j.ygeno.2019.04.003

PMID:30951801

Abstract

TaqMan Low-Density Array (TLDA) based Real-Time PCR (RT-PCR) of selected genes showed increased expression of polycyclic aromatic hydrocarbons (PAHs) metabolizing cytochrome P450s (CYPs), glutathione S-transferases (GSTs) and associated transcription factors in biopsy and peripheral blood samples isolated from head and neck squamous cell carcinoma (HNSCC) patients when compared to the controls. The genes involved in DNA repair, signal transduction pathway, EMT pathway, apoptosis, and cell adhesion/motility were found to be altered in both peripheral blood and biopsy samples of HNSCC patients. Transcription profiles in blood isolated from auto/taxi drivers, with pre-neoplastic lesions and history of tobacco use, also showed similar alterations. The present TLDA data thus demonstrates that low-density array of selected genes in peripheral blood has the potential to be used as a surrogate for providing insight into cancer progression pathways and possibly as an early biomarker for monitoring tobacco induced HNSCC.

摘要

基于 TaqMan 低密度阵列（TLDA）的实时聚合酶链反应（RT-PCR）对选定基因的检测显示，与对照组相比，头颈部鳞状细胞癌（HNSCC）患者活检和外周血样本中多环芳烃（PAHs）代谢细胞色素 P450s（CYPs）、谷胱甘肽 S-转移酶（GSTs）和相关转录因子的表达增加。在 HNSCC 患者的外周血和活检样本中发现涉及 DNA 修复、信号转导途径、上皮间质转化途径、细胞凋亡和细胞黏附/运动的基因发生改变。有癌前病变且有吸烟史的出租车/私家车司机的血液转录谱也显示出类似的改变。因此，本 TLDA 数据表明，外周血中选定基因的低密度阵列具有作为提供癌症进展途径见解的替代物的潜力，并且可能作为监测烟草诱导的 HNSCC 的早期生物标志物。