Ferosekhan Shajahan, Turkmen Serhat, Xu Hanlin, Afonso Juan Manuel, Zamorano Maria Jesus, Kaushik Sadasivam, Izquierdo Marisol
Aquaculture Research Group (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, 35214 Telde, Spain.
ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar 751002, India.
Life (Basel). 2020 Jul 19;10(7):117. doi: 10.3390/life10070117.
The principle aim of this study is to elucidate the relationship between the fatty acid desaturase 2 gene () expression pattern in peripheral blood cells (PBCs) and liver of gilthead seabream (GSB), broodstock in order to determine the possible use of expression as a potential biomarker for the selection of broodstock. This selection could be utilized for breeding programs aiming to improve reproduction, health, and nutritional status. Passive Integrated Transponder (PIT)-tagged GSB broodstock (Male-1.22 ± 0.20 kg; 44.8 ± 2 cm and female-2.36 ± 0.64 kg; 55.1 cm) were fed a diet containing low levels of fish meal and fish oil (EPA 2.5; DHA 1.7 and n-3 LC-PUFA 4.6% TFA) for one month. After the feeding period, expression in PBCs and liver of both male and female broodstock were highly significantly correlated (r = 0.89; < 0.001). Additionally, in male broodstock, liver expression was significantly correlated ( < 0.05) to liver contents in 16:0 (r = 0.95; = 0.04) and total saturates (r = 0.97; = 0.03) as well as to 20:3n-6/20:2n-6 (r = 0.98; = 0.02) a Fads2 product/precursor ratio. Overall, we found a positive and significant correlation between expression levels in the PBCs and liver of GSB broodstock. PBCs expression levels indicate a strong potential for utilization as a non-invasive method to select animals having increased fatty acid bioconversion capability, better able to deal with diets free of fish meal and fish oil.
本研究的主要目的是阐明金头鲷（GSB）亲鱼外周血细胞（PBCs）和肝脏中脂肪酸去饱和酶2基因（）的表达模式之间的关系，以确定该基因表达作为亲鱼选择潜在生物标志物的可能性。这种选择可用于旨在改善繁殖、健康和营养状况的育种计划。对带有被动集成应答器（PIT）标签的GSB亲鱼（雄性-1.22±0.20千克；44.8±2厘米，雌性-2.36±0.64千克；55.1厘米）投喂含有低水平鱼粉和鱼油（EPA 2.5；DHA 1.7，n-3 LC-PUFA 4.6%总脂肪酸）的饲料，为期一个月。投喂期结束后，雄性和雌性亲鱼的PBCs和肝脏中的基因表达高度显著相关（r = 0.89；<0.001）。此外，在雄性亲鱼中，肝脏基因表达与肝脏中16:0（r = 0.95；= 0.04）和总饱和脂肪酸（r = 0.97；= 0.03）的含量以及20:3n-6/20:2n-6（r = 0.98；= 0.02）（一种Fads2产物/前体比率）显著相关（<0.05）。总体而言，我们发现GSB亲鱼的PBCs和肝脏中的基因表达水平之间存在正相关且显著相关。PBCs基因表达水平表明，作为一种非侵入性方法来选择具有增强脂肪酸生物转化能力、更能适应无鱼粉和鱼油饲料的动物具有很大潜力。
