Qin Yinyin, Wang Shiping, Duan Xuchu, Liu Dong
Suqian First Hospital, Suqian, China; School of Life Science, Co-innovation Center of Neuroregeneration, Key Laboratory of Neurogeneration of Jiangsu and MOE, Nantong University, Nantong, China.
Division of Gastrointestinal Surgery, Department of General Surgery, First Affiliated Hospital, Nanjing Medical University, Nanjing, China; Suqian First Hospital, Suqian, China.
Gene Expr Patterns. 2019 Jun;32:38-43. doi: 10.1016/j.gep.2019.04.001. Epub 2019 Apr 2.
The aquaporins are integral membrane proteins from a larger family of major intrinsic protein (MIP) that form pores in the membrane of cells. These proteins selectively transport water and other small uncharged solutes across cell plasma membranes. The organization of water within cells and tissues is fundamental to life, and the aquaporins play an important role in serving as the plumbing system for cells. As many as thirteen mammalian AQPs have been characterized, which have been shown to be vital for the regulation of water homeostasis in most tissues, such as renal water balance and brain-fluid homeostasis. However, complete expression patterns of most of the aquaporins in lower vertebrate at embryo stages has not been elucidated. Currently, we systematically described the temporal-spatial expression pattern of nine zebrafish aquaporins, using whole amount in situ hybridization. The results of whole mount in situ hybridization revealed that members of aquaporins family displayed diverse expression pattern, each of aquaporins has its unique distribution in different cell types and tissues, suggesting that they might play distinct roles in the embryonic development. Overall, current study will provide new insight into the expression of vertebrate quaporins and an important basis for the functional analysis of aquaporins in zebrafish development.
水通道蛋白是主要内在蛋白（MIP）大家族中的整合膜蛋白，可在细胞膜上形成孔道。这些蛋白质选择性地跨细胞质膜转运水和其他不带电荷的小分子溶质。细胞和组织内水的组织形式是生命的基础，而水通道蛋白在作为细胞的管道系统方面发挥着重要作用。已鉴定出多达13种哺乳动物水通道蛋白，它们对大多数组织中的水平衡调节至关重要，如肾脏水平衡和脑液平衡。然而，大多数水通道蛋白在低等脊椎动物胚胎阶段的完整表达模式尚未阐明。目前，我们利用全量原位杂交技术系统地描述了9种斑马鱼水通道蛋白的时空表达模式。全胚原位杂交结果显示，水通道蛋白家族成员表现出多样的表达模式，每种水通道蛋白在不同细胞类型和组织中都有其独特的分布，这表明它们可能在胚胎发育中发挥不同的作用。总体而言，当前研究将为脊椎动物水通道蛋白的表达提供新的见解，并为斑马鱼发育过程中水通道蛋白的功能分析提供重要依据。
