Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, PR China.
Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, Zhengzhou 450002, PR China.
Biosens Bioelectron. 2019 Jun 1;134:8-15. doi: 10.1016/j.bios.2019.03.043. Epub 2019 Mar 22.
We report here a new bimetallic ZrHf metal-organic framework (ZrHf-MOF) embedded with abundant carbon dots (CDs) (denoted as CDs@ZrHf-MOF), which exhibits strong fluorescence and rich-amino-functionalization. The CDs@ZrHf-MOF can be applied as the scaffold for anchoring aptamer strands to determine human epidermal growth factor receptor-2 (HER2) and living HER2-overexpressed MCF-7 cells. The basic characterizations reveal that the CDs are embedded within the interior cavities of ZrHf-MOF without varying the nanostructure, leading to good biocompatibility, strong fluorescence, and high electrochemical activity of CDs@ZrHf-MOF. As compared with the pristine ZrHf-MOF, the CDs@ZrHf-MOF-based electrochemical aptasensor displays better sensing performances toward both HER-2 and MCF-7 cells, giving an extremely low detection limit of 19 fg mL (HER2 concentration range: 0.001-10 ng mL) and 23 cell mL (cell concentration range: 1 × 10~1 × 10 cell mL), with good selectivity, stability, reproducibility, and acceptable applicability. The proposed strategy for developing CDs@ZrHf-MOF-based aptasensor is promising for the early and sensitive detection of cancer markers and living cancer cells.
我们在此报告了一种新型的双金属 ZrHf 金属有机骨架（ZrHf-MOF），其中嵌入了丰富的碳点（CDs）（表示为 CDs@ZrHf-MOF），具有强荧光和丰富的氨基官能化。CDs@ZrHf-MOF 可用作固定适体链的支架，以测定人表皮生长因子受体-2（HER2）和活的 HER2 过表达 MCF-7 细胞。基本特性表明，CDs 嵌入 ZrHf-MOF 的内部腔室中，而不会改变纳米结构，从而导致 CDs@ZrHf-MOF 具有良好的生物相容性、强荧光和高电化学活性。与原始的 ZrHf-MOF 相比，基于 CDs@ZrHf-MOF 的电化学适体传感器对 HER-2 和 MCF-7 细胞均表现出更好的传感性能，对 HER2 的检测下限低至 19 fg mL（HER2 浓度范围：0.001-10 ng mL）和 23 个细胞 mL（细胞浓度范围：1×10~1×10 个细胞 mL），具有良好的选择性、稳定性、重现性和可接受的适用性。基于开发 CDs@ZrHf-MOF 的适体传感器的策略有望用于癌症标志物和活癌细胞的早期和灵敏检测。
