Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, Zhengzhou, 450002, PR China.
Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, Zhengzhou, 450002, PR China.
Biosens Bioelectron. 2019 Oct 1;142:111536. doi: 10.1016/j.bios.2019.111536. Epub 2019 Jul 25.
Combining different metal-organic frameworks (MOFs) into a conjugate material can integrate the properties of each MOF component and further lead to emergent properties from the synergistic heterostructured units. In this work, two kinds of bimetallic TbFe-MOFs have been designed by MOF-on-MOF strategy and utilized as a platform for anchoring carbohydrate antigen 125 (CA125) aptamer to detect CA125 and living michigan cancer foundation-7 (MCF-7) cells. Although the integrated MOF-on-MOF architectures show similar chemical and structural features to that of the top layer, the Fe-MOF-on-Tb-MOF and Tb-MOF-on-Fe-MOF have different surface nanostructures to their parent MOFs. The developed aptasensor based on Tb-MOF-on-Fe-MOF displays higher stability of the formed G-quadruplex between aptamer and CA125 than that based on Fe-MOF-on-Tb-MOF, owing to stronger immobilization behavior of the aptamer for the Tb-MOF-on-Fe-MOF composite. The developed aptasensor provides an extremely low detection limit of 58 μU·mL towards CA125 within a wide linear range from 100 μU·mL to 200 U·mL, which is significantly lower than those of all reported sensors. This aptasensor also has high selectivity, good stability, acceptable reproducibility, and excellent applicability in human serum. Moreover, the Tb-MOF-on-Fe-MOF nanoarchitecture demonstrates superior biocompatibility and good endocytosis. As a result, the developed aptasensor illustrates high sensitivity for detection of MCF-7 cells with an extremely low detection limit of 19 cell·mL. Therefore, the proposed aptasensor based on Tb-MOF-on-Fe-MOF exhibits great potentials for early diagnosis of tumors.
将不同的金属有机骨架（MOFs）组合成一种共轭材料可以整合每个 MOF 组件的性质，并进一步导致协同异质结构单元产生新的性质。在这项工作中，通过 MOF-on-MOF 策略设计了两种双金属 TbFe-MOF，并将其用作固定碳水化合物抗原 125（CA125）适体的平台，以检测 CA125 和活密歇根癌症基金会-7（MCF-7）细胞。尽管集成的 MOF-on-MOF 结构在化学和结构特征上与顶层相似，但 Fe-MOF-on-Tb-MOF 和 Tb-MOF-on-Fe-MOF 具有与其母体 MOFs 不同的表面纳米结构。基于 Tb-MOF-on-Fe-MOF 的开发的适体传感器与基于 Fe-MOF-on-Tb-MOF 的传感器相比，具有更高的适体与 CA125 之间形成的 G-四链体的稳定性，这是由于适体对 Tb-MOF-on-Fe-MOF 复合材料具有更强的固定化行为。开发的适体传感器对 CA125 的检测限低至 58μU·mL，线性范围从 100μU·mL 到 200U·mL 很宽，明显低于所有报道的传感器。该适体传感器还具有高选择性、良好的稳定性、可接受的重现性以及在人血清中的出色适用性。此外，Tb-MOF-on-Fe-MOF 纳米结构具有优异的生物相容性和良好的内吞作用。结果，开发的适体传感器对 MCF-7 细胞具有高灵敏度，检测限低至 19 细胞·mL。因此，基于 Tb-MOF-on-Fe-MOF 的开发的适体传感器在肿瘤的早期诊断方面具有很大的潜力。
