Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain.
Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain.
Talanta. 2019 Jul 1;199:262-269. doi: 10.1016/j.talanta.2019.02.061. Epub 2019 Feb 16.
The World Health Organization considers iodide deficiency diseases (IDD) to be a public health problem. The main indicator to access IDD is urinary iodide, since approximately 90% of the ingested iodide uses this clearance path, with urine being a preferable target for the analysis. In this work, two screen-printed carbon electrode (SPCE) based sensors were developed to determine iodide by using only a single drop of sample. A first approach based on a SPCE proves to selectively determine iodide through the control of the cathodic stripping voltammetric (CSV) parameters. However, this strategy exhibits a gap in determining trace iodide concentrations, which is improved by modifying the working electrode surface with a chitosan coating. The performance of this new CS/SPCE-based sensor was compared with that of the previous SPCE-based sensor, showing improved iodide determination sensitivity. A limit of detection of 1.0 × 10 M and a linear analysis range of 0.15-500 µM were achieved with this sensor. The application of both sensors to real-life samples found values close to those determined by the standard Sandell-Kolthoff spectrophotometric method, proving them to be powerful analytical tools for iodide determination in different kinds of samples, including biological matrices.
世界卫生组织认为碘缺乏病（IDD）是一个公共卫生问题。评估碘缺乏病的主要指标是尿碘，因为大约 90%的摄入碘通过这种清除途径，尿液是分析的首选目标。在这项工作中，开发了两种基于丝网印刷碳电极（SPCE）的传感器，仅使用单个样本滴即可确定碘化物。第一种方法基于 SPCE，通过控制阴极剥离伏安法（CSV）参数来选择性地确定碘化物。然而，该策略在确定痕量碘化物浓度方面存在差距，通过用壳聚糖涂层修饰工作电极表面来改善这一问题。新的 CS/SPCE 基传感器的性能与之前的基于 SPCE 的传感器进行了比较，显示出提高的碘化物测定灵敏度。该传感器的检测限为 1.0×10 M，线性分析范围为 0.15-500 µM。两种传感器在实际样品中的应用得到了与标准桑德尔-科尔托夫分光光度法测定值接近的值，证明它们是不同类型样品中碘化物测定的有力分析工具，包括生物基质。
