Central Laboratory, Careggi University Hospital, Florence, Italy.
Department of Experimental and Clinical Medicine, University of Florence, Italy.
Nutr Metab Cardiovasc Dis. 2019 Jun;29(6):604-610. doi: 10.1016/j.numecd.2019.02.005. Epub 2019 Feb 26.
To evaluate the possible association between dietary habits and progenitor cells using data obtained from a randomized crossover trial using two different diets, lacto-ovo-vegetarian (VD) and Mediterranean (MD), the CARDIVEG study.
Eighty clinically healthy subjects with a low-to-moderate cardiovascular risk profile (61 F; 19 M; mean age: 50.7 ± 11.6 years) were randomly assigned to isocaloric VD and MD diets lasting three months each, and then crossed. The two diets showed no effects on endothelial progenitor cells and circulating endothelial cells but opposite effects on circulating progenitor cells. In fact, VD determined significant (p < 0.05) and negative changes on circulating progenitor cells, with an average geometric variation of -130 cells/10 events for CD34/CD45, -80 cells/10 events for CD133/CD45, and -84 cells/10 events for CD34/CD133/CD45 while MD determined significant (p < 0.05) and positive changes for CD34/CD45 levels, with a geometric mean increase of +54 cells/10 events. No significant correlations were observed between changes in progenitor cells and changes in inflammatory parameters during the VD phase. On the other hand, during the MD phase negative correlations between changes of CD34/CD45 and interleukin-6 (R = -0.324; p = 0.004) as well as interleukin-8 (R = -0.228; p = 0.04) and monocyte chemotactic protein-1 (R = -0.277; p = 0.01), were observed. These correlations remained significant also after adjustment for confounding factors only for CD34/CD45 and interleukin-6 (β = -0.282; p = 0.018) and monocyte chemotactic protein-1 (β = -0.254; p = 0.031).
MD, but not VD, reported a significant and positive effect on circulating progenitor cells in a group of subjects at low-to-moderate cardiovascular risk, probably acting through the modulation of inflammatory parameters.
使用两种不同饮食（乳蛋素食（VD）和地中海饮食（MD））的随机交叉试验获得的数据，评估饮食习惯与祖细胞之间可能存在的关联，该研究为 CARDIVEG 研究。
80 名临床健康、低至中度心血管风险的受试者（61 名女性；19 名男性；平均年龄：50.7±11.6 岁）被随机分配至热量相同的 VD 和 MD 饮食组，每组持续 3 个月，然后交叉。两种饮食对内皮祖细胞和循环内皮细胞均无影响，但对循环祖细胞有相反的影响。事实上，VD 对循环祖细胞产生显著（p<0.05）且负面的变化，CD34/CD45 的平均几何变化为-130 个细胞/10 事件，CD133/CD45 为-80 个细胞/10 事件，CD34/CD133/CD45 为-84 个细胞/10 事件，而 MD 对 CD34/CD45 水平产生显著（p<0.05）且正面的变化，几何平均增加 54 个细胞/10 事件。在 VD 期间，未观察到祖细胞变化与炎症参数变化之间存在显著相关性。另一方面，在 MD 期间，CD34/CD45 与白细胞介素-6（R=-0.324；p=0.004）和白细胞介素-8（R=-0.228；p=0.04）以及单核细胞趋化蛋白-1（R=-0.277；p=0.01）之间呈负相关。这些相关性在仅调整混杂因素后仍然显著，仅对 CD34/CD45 和白细胞介素-6（β=-0.282；p=0.018）和单核细胞趋化蛋白-1（β=-0.254；p=0.031）有意义。
在低至中度心血管风险的受试者中，MD 而非 VD 饮食对循环祖细胞有显著且正面的影响，可能通过调节炎症参数起作用。
