具有上皮-间质转化表型的循环肿瘤细胞与原发性乳腺癌较差的预后相关。
Circulating Tumor Cells With Epithelial-to-mesenchymal Transition Phenotypes Associated With Inferior Outcomes in Primary Breast Cancer.
作者信息
Mego Michal, Karaba Marian, Minarik Gabriel, Benca Juraj, Silvia Jurisova, Sedlackova Tatiana, Manasova Denisa, Kalavska Katarina, Pindak Daniel, Cristofanilli Massimo, Reuben James M, Mardiak Jozef
机构信息
2nd Department of Oncology, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic
National Cancer Institute, Bratislava, Slovak Republic.
出版信息
Anticancer Res. 2019 Apr;39(4):1829-1837. doi: 10.21873/anticanres.13290.
BACKGROUND/AIM: Circulating tumor cells (CTCs) comprise a heterogeneous population of cancer cells with different clinical and biological value. The aim of this study was to evaluate the prognostic value of CTCs with an epithelial-mesenchymal transition (EMT) phenotype in primary breast cancer (PBC) patients.
PATIENTS AND METHODS
This study included 427 primary breast cancer patients. RNA extracted from CD45-depleted peripheral blood mononuclear cell (PBMCs) was evaluated for the expression of EMT transcription factors (TWIST1, SNAIL1, SLUG, ZEB1) by quantitative real time polymerase chain reaction (qRT-PCR).
RESULTS
In total, CTC EMT was detected in 77 (18.0%) patients. Patients without detectable CTC EMT in peripheral blood had significantly longer disease-free survival than patients with detectable CTC EMT. The prognostic value of CTC EMT was demonstrated in all subgroups of patients.
CONCLUSION
CTCs with an EMT phenotype have a prognostic value in primary breast cancer.
背景/目的：循环肿瘤细胞（CTC）由具有不同临床和生物学价值的异质性癌细胞群体组成。本研究的目的是评估具有上皮-间质转化（EMT）表型的CTC在原发性乳腺癌（PBC）患者中的预后价值。
患者与方法
本研究纳入了427例原发性乳腺癌患者。通过定量实时聚合酶链反应（qRT-PCR）评估从去除CD45的外周血单个核细胞（PBMC）中提取的RNA中EMT转录因子（TWIST1、SNAIL1、SLUG、ZEB1）的表达。
结果
总共在77例（18.0%）患者中检测到CTC EMT。外周血中未检测到CTC EMT的患者无病生存期明显长于检测到CTC EMT的患者。在所有患者亚组中均证实了CTC EMT的预后价值。
结论
具有EMT表型的CTC在原发性乳腺癌中具有预后价值。
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