芫花素诱导人膀胱癌TSGH-8301细胞的DNA损伤并损害DNA修复。

Casticin Induces DNA Damage and Impairs DNA Repair in Human Bladder Cancer TSGH-8301 Cells.

作者信息

Huang An-Cheng, Cheng Yih-Dih, Huang Li-Hsueh, Hsiao Yung-Ting, Peng Shu-Fen, Lu Kung-Wen, Lien Jin-Cherng, Yang Jong-Long, Lin Tzu-Shun, Chung Jing-Gung

机构信息

Department of Nursing, St. Mary's Junior College of Medicine, Nursing and Management, Sanxing, Taiwan, R.O.C.

Department of Pharmacy, China Medical University Hospital, Taichung, Taiwan, R.O.C.

出版信息

Anticancer Res. 2019 Apr;39(4):1839-1847. doi: 10.21873/anticanres.13291.

DOI:10.21873/anticanres.13291

PMID:30952724

Abstract

BACKGROUND/AIM: Casticin shows anti-cancer effects in many types of cancer. However, there is no information regarding its role in DNA damage in human bladder cancer. The aim of this study was to investigate the effects of casticin on TSGH-8301 cells in vitro.

MATERIALS AND METHODS

Viability of cells was assayed by flow cytometry. DNA damage was assayed by DAPI staining, comet assay, and gel electrophoresis. Protein levels were examined by western blotting and confocal laser microscopy.

RESULTS

Casticin decreased viability of cells and induced DNA damage. Furthermore, casticin decreased expression of p-ATM, p-ATR, MDC1 and MGMT levels after 48 h of treatment, however, it increased p-ATR and MGMT levels after 12 h. In contrast, casticin increased the levels of p-p53, p-H2A.X, and PARP after 48 h of treatment. As shown by confocal microscopy, casticin affected the translocation of DNA-PKcs and p-p53 to the nucleus of TSGH-8301 cells.

CONCLUSION

Casticin decreased viability of human bladder cancer cells through DNA damage.

摘要

背景/目的：芫花素在多种癌症中显示出抗癌作用。然而，关于其在人膀胱癌DNA损伤中的作用尚无相关信息。本研究的目的是在体外研究芫花素对TSGH-8301细胞的影响。

材料与方法

通过流式细胞术检测细胞活力。通过DAPI染色、彗星试验和凝胶电泳检测DNA损伤。通过蛋白质印迹和共聚焦激光显微镜检查蛋白质水平。

结果

芫花素降低细胞活力并诱导DNA损伤。此外，芫花素在处理48小时后降低了p-ATM、p-ATR、MDC1和MGMT的表达水平，然而，在处理12小时后它增加了p-ATR和MGMT的水平。相反，芫花素在处理48小时后增加了p-p53、p-H2A.X和PARP的水平。如共聚焦显微镜所示，芫花素影响DNA-PKcs和p-p53向TSGH-8301细胞核的转位。