在各大生物群落中，可培养的细菌比例很高。
High proportions of bacteria are culturable across major biomes.
机构信息
Department of Earth System Science, University of California, Irvine, CA, 92697, USA.
Department of Ecology and Evolutionary Biology, University of California, Irvine, CA, 92697, USA.
出版信息
ISME J. 2019 Aug;13(8):2125-2128. doi: 10.1038/s41396-019-0410-3. Epub 2019 Apr 5.
The paradigm that only 1% of microbes are culturable has had a profound impact on our understanding of microbial ecology and is still a major motivation for mostly using molecular tools to characterize microbial communities. However, this point is often expressed vaguely, suggesting that some scientists have different interpretations of the paradigm. In addition, there have been substantial advances in cultivation techniques suggesting that this paradigm may no longer be correct. To quantify bacterial culturability across six major biomes, I found that the median 16S rRNA similarity of bacteria to known cultured relatives was 97.3 ± 2.3% (s.d.). Furthermore, 52.0 ± 24% of sequences and 34.9 ± 23% of taxa (defined as >97% similar) had a closely related cultured relative. Thus, many cells and taxa across environments are culturable with known techniques, suggesting that the 1% paradigm is no longer correct.
只有 1%的微生物可培养的范式对我们理解微生物生态学产生了深远的影响，并且仍然是主要使用分子工具来描述微生物群落的主要动机。然而，这一点常常被模糊地表达出来，表明一些科学家对该范式有不同的解释。此外，培养技术有了实质性的进步，表明这一范式可能不再正确。为了量化六个主要生物群落中的细菌可培养性，我发现细菌与已知培养相关物的 16S rRNA 相似性中位数为 97.3±2.3%（标准差）。此外，52.0±24%的序列和 34.9±23%的分类单元（定义为>97%相似）有密切相关的培养相关物。因此，许多环境中的细胞和分类单元都可以用已知技术进行培养，这表明 1%的范式不再正确。
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