Department of Veterinary Science, Federal University of Paraná, Curitiba, PR, Brazil.
Department of Animal Science, Federal University of Paraná, Curitiba, PR, Brazil.
Poult Sci. 2019 Sep 1;98(9):3989-3993. doi: 10.3382/ps/pez141.
Small eggs have lesser amounts of nutrients to be used by the embryo, and the yolk glycerol is the main substrate for glycogen production, which is the main energy source in the last days of incubation. Thus, the present study aimed to assess the effect of a glycerol injection in light weight eggs at 2 different days of incubation. To this end, 336 light eggs (55.6 to 58.6 g) from 32-wk-old broiler breeders were incubated. The eggs were divided into 3 treatment groups: 1 group inoculated with saline solution on the 17th d of embryonic development (E17) (control group), the second group injected with a 6 mg glycerol/mL solution at E17, and the third group injected with 6 mg glycerol/mL on the 18th d of incubation (E18). Incubation parameters, liver and muscle glycogen, and broilers performance at 7 d of age were evaluated. Glycerol administration in ovo did not influence hatchability, period of embryonic death or early hatching. Chicks exposed to glycerol in ovo feeding (IOF) used more yolk than birds inoculated with saline solution. Glycerol inoculation at E18 enhanced liver glycogen deposition (P = 0.001) and also improved broilers performance at 7 d, although this improvement in performance and glycogen reserves was not observed when eggs were inoculated at 17 d of incubation. Birds receiving glycerol IOF at E18 showed higher feed intake and body weight gain when compared to the control group and the group inoculated at E17. It was found that glycerol inoculation in light eggs at the 18th d of incubation contributed to raise liver glycerol levels and also to improve broilers performance at 7 d.
小蛋的胚胎可利用的营养物质较少，蛋黄甘油是糖原产生的主要基质，糖原是孵化最后几天的主要能量来源。因此，本研究旨在评估在孵化的第 2 天向轻重量蛋中注射甘油的效果。为此，将 336 个来自 32 周龄肉鸡种鸡的轻蛋（55.6 至 58.6 克）进行孵化。这些蛋被分为 3 个处理组：1 组在胚胎发育的第 17 天（E17）接种生理盐水（对照组），第 2 组在 E17 时注射 6 毫克甘油/毫升溶液，第 3 组在孵化的第 18 天（E18）注射 6 毫克甘油/毫升。评估孵化参数、肝脏和肌肉糖原以及 7 日龄肉鸡的性能。卵内注射甘油对孵化率、胚胎死亡期或早期孵化没有影响。暴露于卵内甘油喂养（IOF）的雏鸡比接种生理盐水的雏鸡消耗更多的蛋黄。E18 时甘油接种增强了肝脏糖原沉积（P=0.001），并提高了 7 日龄肉鸡的性能，尽管在孵化第 17 天接种时，这种性能和糖原储备的改善没有观察到。与对照组和 E17 接种组相比，E18 时接受 IOF 甘油的雏鸡表现出更高的采食量和体重增加。结果表明，在孵化的第 18 天向轻蛋中注射甘油有助于提高肝脏甘油水平，并提高 7 日龄肉鸡的性能。
