Eray Oğuzhan, Filik Gökhan
Biotechnology Research Centre, Central Research Institute for Field Crops, Research and Technology Development Campus, Yenimahalle, Ankara, Türkiye.
Department of Agricultural Biotechnology, Faculty of Agriculture, University of Kırşehir Ahi Evran, Kırşehir, Türkiye.
Vet Med Sci. 2025 Jan;11(1):e70153. doi: 10.1002/vms3.70153.
This study aimed to determine the effects of in ovo formula product injection on hatching parameters, chick quality, small intestinal development and ileum histology of breeder hen eggs.
A total of 400 fertilised eggs were obtained from the Atak-S parent flock at 42 weeks of age for the experiment. The experiment was designed in two groups: a control group (C), in which no injection was performed, and the other group in which a solution containing formula products at concentrations of 1.25% (F1), 2.5% (F2) and 5% (F3) was injected into 0.5 mL/egg air sac. The hatching rate, embryonic mortality and discard chick rate were examined at the end of the trial.
The best result of the hatching rate was found in the Group F2, while there was no difference between the control and Group F1 regarding these parameters. The weight and length of the quality chicks were promoted in the Group F1. In Group F3, the injection of the formula product at a rate of 5% had a negative effect on parameters such as hatchability, embryonic mortality, chick length, Pasgar score and yolk sac weight compared to the other groups. Otherwise, in Group F2, there was a significant increase in villus height, crypt depth and lamina muscularis mucosa thickness compared to the other groups (p < 0.01).
As a result, it has been concluded that the appropriate rates for the formula product application on chicks are 1.25% and 2.5%, considering the positive effects of the 1.25% and 2.5% rates and the negative effects of the 5% rate.
本研究旨在确定蛋内注射配方产品对种母鸡种蛋孵化参数、雏鸡质量、小肠发育及回肠组织学的影响。
从42周龄的Atak-S父母代鸡群中获取400枚受精蛋用于实验。实验分为两组：对照组（C），不进行注射；另一组，将浓度为1.25%（F1）、2.5%（F2）和5%（F3）的配方产品溶液以0.5 mL/蛋的剂量注入气室。试验结束时检查孵化率、胚胎死亡率和淘汰雏鸡率。
F2组的孵化率最佳，而对照组和F1组在这些参数上无差异。F1组优质雏鸡的体重和长度增加。与其他组相比，F3组以5%的比例注射配方产品对孵化率、胚胎死亡率、雏鸡长度、帕斯卡评分和卵黄囊重量等参数有负面影响。此外，与其他组相比，F2组的绒毛高度、隐窝深度和黏膜肌层厚度显著增加（p < 0.01）。
因此，考虑到1.25%和2.5%比例的积极作用以及5%比例的消极作用，得出在雏鸡上应用配方产品的适宜比例为1.25%和2.5%的结论。
