College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou, Jiangsu, China.
J Cell Physiol. 2019 Nov;234(11):20329-20341. doi: 10.1002/jcp.28633. Epub 2019 Apr 5.
The hair follicle is a complex biological system involved in a dynamic process governed by gene regulation. MicroRNAs play a critical role in the regulation of gene expression. We demonstrated that the expression of miR-218-5p and SFRP2 showed the opposite relationship in catagen and telogen phases and that miR-218-5p promoted the growth of hair shafts. The luciferase reporter assays confirmed that SFRP2 is the direct target of miR-218-5p. The expression of miR-218-5p may decrease the expression of SFRP2, which activates the Wnt signaling pathway, including the regulation of downstream genes and β-catenin/T-cell-specific factor transcriptional activity. Moreover, miR-218-5p enhanced apoptosis, but inhibition of miR-218-5p decreased apoptosis and inhibited RAB-9 cell proliferation. In this study, we show that miR-218-5p positively regulates the Wnt signaling pathway by targeting SFRP2 and acts as a dynamic governor during skin and hair follicle development.
毛囊是一个复杂的生物系统，参与由基因调控的动态过程。MicroRNAs 在基因表达调控中发挥着关键作用。我们证明，miR-218-5p 和 SFRP2 的表达在退行期和休止期呈相反的关系，并且 miR-218-5p 促进毛干的生长。荧光素酶报告基因检测证实 SFRP2 是 miR-218-5p 的直接靶标。miR-218-5p 的表达可能降低 SFRP2 的表达，从而激活 Wnt 信号通路，包括下游基因和β-连环蛋白/T 细胞特异性因子转录活性的调节。此外，miR-218-5p 增强细胞凋亡，而抑制 miR-218-5p 则减少细胞凋亡并抑制 RAB-9 细胞增殖。在这项研究中，我们表明 miR-218-5p 通过靶向 SFRP2 正向调节 Wnt 信号通路，并在皮肤和毛囊发育过程中充当动态调控因子。
