Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Jinan 250100, China.
Cells. 2024 Mar 21;13(6):557. doi: 10.3390/cells13060557.
Wool is produced and controlled by hair follicles (HFs). However, little is known about the mechanisms involved in HF development and regulation. Sheep dermal fibroblasts (SDFs) play a key role in the initial stage of HF development. Analyzing the molecular mechanism that regulates early HF development in superfine wool sheep is of great importance for better understanding the HF morphogenesis process and for the breeding of fine wool sheep. Here, we show that two microRNAs (miRNAs) affect the development of HFs by targeting two genes that are expressed by SDFs. Meanwhile, the overexpression and inhibition of oar-miR-23b and oar-miR-133 in SDFs cells and cell proliferation, apoptosis, and migration were further detected using a CCK-8 assay, an Annexin V-FITC assay, a Transwell assay, and flow cytometry. We found that oar-miR-23b, oar-miR-133, and their cotarget genes and were differentially expressed during the six stages of HF development in superfine wool sheep. Oar-miR-23b and oar-miR-133 inhibited the proliferation and migration of SDFs and promoted the apoptosis of SDFs through and . oar-miR-23b and oar-miR-133 inhibited the proliferation and migration of SDFs by jointly targeting and , thereby inhibiting the development of superfine wool HFs. Our research provides a molecular marker that can be used to guide the breeding of ultrafine wool sheep.
羊毛由毛囊（HFs）产生和控制。然而，人们对 HF 发育和调节所涉及的机制知之甚少。绵羊真皮成纤维细胞（SDFs）在 HF 发育的初始阶段发挥关键作用。分析调控超细羊毛羊早期 HF 发育的分子机制，对于更好地理解 HF 形态发生过程和培育细毛羊具有重要意义。在这里，我们表明，两种 microRNAs（miRNAs）通过靶向 SDFs 表达的两个基因来影响 HF 的发育。同时，通过 CCK-8 测定、Annexin V-FITC 测定、Transwell 测定和流式细胞术进一步检测了 oar-miR-23b 和 oar-miR-133 在 SDFs 细胞中的过表达和抑制作用以及细胞增殖、凋亡和迁移。我们发现 oar-miR-23b、oar-miR-133 及其共同靶基因 和 在超细羊毛羊 HF 发育的六个阶段中差异表达。oar-miR-23b 和 oar-miR-133 通过 和 抑制 SDFs 的增殖和迁移，并促进 SDFs 的凋亡。oar-miR-23b 和 oar-miR-133 通过共同靶向 和 抑制 SDFs 的增殖和迁移，从而抑制超细羊毛 HF 的发育。我们的研究为指导超细羊毛羊的选育提供了一个分子标记。
