Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China.
College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
Int J Biol Macromol. 2019 Jul 1;132:801-810. doi: 10.1016/j.ijbiomac.2019.04.015. Epub 2019 Apr 3.
Iron is essential in fundamental bioactivities, so it makes sense to improve the efficiency of iron on epithelial transport. In this work, a novel polysaccharide‑iron(III) complex (FVP-Fe(III)) was prepared from Flammulina velutipes with a specific structure. The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9. In the vitro digestion model, the complex could maintain better solubility and steady of iron than FeSO. In the cell assay, FVP-Fe(III) showed lower cytotoxicity and better absorption. The transport amount of FVP-Fe(III) was 1.5-fold of FeSO at same concentration and 1.8-fold of FeSO at same time. The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter. The results of this study suggested that the polysaccharide obtained from F. velutipes could be developed a new kind of iron delivery for further study.
铁在基本的生物活性中是必不可少的，因此提高铁对上皮细胞转运的效率是有意义的。在这项工作中，从金针菇中制备了一种具有特定结构的新型多糖-铁（III）复合物（FVP-Fe(III)）。FVP-Fe(III) 的分子量为 15.13 kDa，其单糖组成是甘露糖、半乳糖醛酸、葡萄糖、半乳糖、木糖、岩藻糖，摩尔比为 3.6:2.1:60.8:18.7:3.9:10.9。在体外消化模型中，该复合物比 FeSO 能更好地保持铁的溶解度和稳定性。在细胞测定中，FVP-Fe(III) 表现出较低的细胞毒性和更好的吸收性。在相同浓度下，FVP-Fe(III) 的转运量是 FeSO 的 1.5 倍，在相同时间内，FVP-Fe(III) 的转运量是 FeSO 的 1.8 倍。这种转运是由肽转运体（pepT1）主动转运途径介导的，样品的外排主要由多药耐药蛋白（MRP）转运体介导。本研究结果表明，金针菇中获得的多糖可以开发成一种新的铁传递体，以进一步研究。
