Zhao Huimin, Zang Lixin, Xu Kehua, Kou Meng, Zhang Zhiguo
Shandong Provincial Engineering and Technical Center of Light Manipulations, Shandong Provincial Key Laboratory of Optics and Photonic Device, School of Physics and Electronics, College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Ji'nan 250014, China.
Shandong Provincial Engineering and Technical Center of Light Manipulations, Shandong Provincial Key Laboratory of Optics and Photonic Device, School of Physics and Electronics, College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Ji'nan 250014, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jun 15;217:310-314. doi: 10.1016/j.saa.2019.03.107. Epub 2019 Mar 29.
High oxygen sensitivity (the slope of the Stern-Volmer plot reaches 0.73/μM) is achieved with a phosphorescence indicator, gadolinium-hematoporphyrin monomethyl ether (Gd-HMME), by decreasing the extent of its protection. In air-saturated solution, the phosphorescence quantum efficiency (QE) of Gd-HMME in a non-rigid microenvironment is lower than that in a rigid microenvironment. In contrast, when oxygen is removed, the QE of Gd-HMME in the non-rigid microenvironment was found to be same as that of Gd-HMME in the rigid microenvironment. This indicates that Gd-HMME is much more sensitive to oxygen in the non-rigid microenvironment. The oxygen sensitivity of Gd-HMME was found to increase as the rigidity of its microenvironment decreases. The oxygen response of Gd-HMME without any protection reaches 240 (0-374 μM oxygen), whereas that in the rigid microenvironment is only 3 in this range. The measurement precision of Gd-HMME without any protection is lower than that in the rigid microenvironment. These results indicate that the measurement of oxygen in different concentration ranges would require the rigidity of the microenvironment to be varied. Gd-HMME without any protection can be applied to detect oxygen as low as 0.1 μM. The detection limit of oxygen was evaluated to be as low as 20 nM based on Gd-HMME without any protection.
通过降低其保护程度，使用磷光指示剂钆-血卟啉单甲醚（Gd-HMME）可实现高氧敏感性（Stern-Volmer图的斜率达到0.73/μM）。在空气饱和溶液中，Gd-HMME在非刚性微环境中的磷光量子产率（QE）低于刚性微环境中的磷光量子产率。相反，当去除氧气时，发现Gd-HMME在非刚性微环境中的QE与刚性微环境中的Gd-HMME相同。这表明Gd-HMME在非刚性微环境中对氧气更为敏感。发现Gd-HMME的氧敏感性随着其微环境刚性的降低而增加。未加任何保护的Gd-HMME的氧响应在0-374μM氧气范围内达到240，而在刚性微环境中此范围内仅为3。未加任何保护的Gd-HMME的测量精度低于刚性微环境中的测量精度。这些结果表明，在不同浓度范围内测量氧气需要改变微环境的刚性。未加任何保护的Gd-HMME可用于检测低至0.1μM的氧气。基于未加任何保护的Gd-HMME，评估出氧气的检测限低至20 nM。
