Liu Ting, Zhang Xiyu, Zhang Honglin, Zhao Hua, Zhang Zhiguo, Tian Ye
Opt Express. 2020 Aug 31;28(18):25757-25766. doi: 10.1364/OE.401423.
The singlet oxygen quantum yield (Φ) was monitored in real time through time resolved spectroscopy measurement, using gadolinium labeled hematoporphyrin monomethyl ether (Gd-HMME) as photosensitizer. According to the kinetics equations of singlet oxygen generation and reaction, Φ was related to phosphorescence lifetime (τ). Through measuring τ of Gd-HMME in different oxygen conditions, the radiation transition property of first exited triplet state (T) was monitored; combined with the triplet state quantum yield (Φ) determined by linear fitting the Φ, which was measured in different oxygen content using a relative measurement, Φ can be determined in real time. The identification of anoxia during the treatment of photodynamic therapy (PDT) by this method is also presented.
使用钆标记的血卟啉单甲醚（Gd-HMME）作为光敏剂，通过时间分辨光谱测量实时监测单线态氧量子产率（Φ）。根据单线态氧生成和反应的动力学方程，Φ与磷光寿命（τ）相关。通过测量不同氧气条件下Gd-HMME的τ，监测第一激发三重态（T）的辐射跃迁特性；结合通过相对测量在不同氧含量下测量的Φ进行线性拟合确定的三重态量子产率（Φ），可以实时确定Φ。还介绍了通过该方法在光动力疗法（PDT）治疗过程中对缺氧的识别。
