Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Fish Diseases and Management Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Environ Toxicol Pharmacol. 2019 Jul;69:44-50. doi: 10.1016/j.etap.2019.03.016. Epub 2019 Mar 18.
Industrial products contained nano-zinc oxide (ZnONP) can gain access to the aquaculture environment causing hazardous effects on the living biota. Therefore, this work was planned to examine the ameliorative effects of dietary supplementation of lycopene (LYC) and/or resveratrol (RES) against ZnONP toxicity in Nile tilapia. Five groups with 20 fish each were used; Control, received tap water only; ZnONP group, was intoxicated with ZnONP (50 mg/L); ZnONP-LYC group, was exposed to ZnONP and LYC (500 mg/ kg of the diet); ZnONP-RES group, was exposed to ZnONP and RES (50 mg/kg of the diet); ZnONP-LYC-RES group, was exposed to ZnONP and a combination of LYC and RES. The experiment was continued for 30 days. Fish blood and tissues were then assembled for determination of liver and kidney function and oxidative stress status in liver, kidney, and gills tissue. Results revealed a considerable elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), cholesterol, urea, and creatinine with a noticeable lowering of total proteins and albumin serum levels in response to ZnONP intoxication. In addition, there were significant increase in malondialdehyde (MDA) and reduction in the reduced-glutathione (GSH) levels and superoxide dismutase (SOD) and catalase (CAT) activities. However, treatment with LYC and/or RES ameliorated the ZnONP-inflicted oxidative stress which possibly attributed to their beneficial antioxidant activities.
工业产品中含有的纳米氧化锌（ZnONP）可能会进入水产养殖环境，对生物产生有害影响。因此，本研究旨在探讨番茄红素（LYC）和/或白藜芦醇（RES）对尼罗罗非鱼（Nile tilapia）ZnONP 毒性的缓解作用。使用了 5 个组，每组 20 条鱼；对照组，仅接受自来水；ZnONP 组，用 ZnONP（50mg/L）处理；ZnONP-LYC 组，用 ZnONP 和 LYC（500mg/kg 饲料）处理；ZnONP-RES 组，用 ZnONP 和 RES（50mg/kg 饲料）处理；ZnONP-LYC-RES 组，用 ZnONP 和 LYC 和 RES 的混合物处理。实验持续了 30 天。然后收集鱼的血液和组织，用于测定肝脏和肾脏功能以及肝脏、肾脏和鳃组织的氧化应激状态。结果表明，与对照组相比，AST、ALT、ALP、胆固醇、尿素和肌酐显著升高，血清总蛋白和白蛋白水平显著降低，这表明 ZnONP 中毒会导致氧化应激。此外，MDA 水平显著升高，GSH 水平显著降低，SOD 和 CAT 活性显著降低。然而，LYC 和/或 RES 的处理缓解了 ZnONP 引起的氧化应激，这可能归因于它们的有益的抗氧化活性。
