Li Han, Cui Xiumei, Zheng Liangyu
Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, P. R. China.
J Microbiol Biotechnol. 2019 Apr 28;29(4):607-616. doi: 10.4014/jmb.1811.11054.
In this study, functionalized poplar powder (FPP) was used as a support material for the immobilization of enoate reductase (ER) and glucose-6-phosphate dehydrogenase (GDH) by covalent binding. Under optimal conditions, the immobilization efficiency of ER-FPP and GDH-FPP was 95.1% and 84.7%, and the activity recovery of ER and GDH was 47.5% and 37.8%, respectively. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) analysis indicated that FPP was a suitable carrier for enzyme immobilization. ER-FPP and GDH-FPP exhibit excellent thermal stabilities and superior reusability. Especially, ER-FPP and GDH-FPP enable the continuous conversion of 4-(4-Methoxyphenyl)-3-buten-2-one with NAD recycling. While the immobilization strategies established here were simple and inexpensive, they exploited a new method for the immobilization and application of ER and its cofactor recycling system.
在本研究中，功能化杨木粉（FPP）被用作通过共价结合固定烯酸酯还原酶（ER）和葡萄糖-6-磷酸脱氢酶（GDH）的载体材料。在最佳条件下，ER-FPP和GDH-FPP的固定化效率分别为95.1%和84.7%，ER和GDH的活性回收率分别为47.5%和37.8%。扫描电子显微镜（SEM）和能量色散光谱（EDS）分析表明，FPP是一种适合酶固定化的载体。ER-FPP和GDH-FPP表现出优异的热稳定性和卓越的可重复使用性。特别是，ER-FPP和GDH-FPP能够通过NAD循环实现4-（4-甲氧基苯基）-3-丁烯-2-酮的连续转化。虽然这里建立的固定化策略简单且成本低廉，但它们开发了一种用于ER及其辅因子循环系统固定化和应用的新方法。
