State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing, 400715, China.
State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing, 400715, China; Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing, 400715, China.
Biochem Biophys Res Commun. 2019 May 21;513(1):280-286. doi: 10.1016/j.bbrc.2019.03.100. Epub 2019 Apr 4.
Transcription factor E93 is a steroid hormone ecdysone early response gene and plays crucial roles in both the degradation of larval tissues and the formation of adult organs during insect metamorphosis with the prepupal-pupal-adult transition. However, the molecular mechanism underlying E93 regulation is poorly understood. In this study, we found that specific knockdown of the E93 gene in the Drosophila wing disrupted wing development. Analyzing ChIP-seq signals for E93 in Drosophila wing identified that the decapentaplegic (Dpp) gene was a potential downstream target of E93. ChIP-PCR analysis and dual-luciferase reporter assay confirmed that E93 could bind to the Dpp promoter and enhanced its activity. Furthermore, the expressions of Dpp and other components in the Dpp signaling pathway were upregulated following E93 overexpression in Drosophila S2 cells but were decreased after E93 knockdown in the wing. Moreover, the impairment of the Dpp signaling pathway phenocopied the defects of E93 knockdown on wing development. Taken together, our results suggest that E93 modulates the Dpp signaling pathway to regulate wing development during Drosophila metamorphosis.
转录因子 E93 是一种类固醇激素蜕皮激素早期反应基因，在昆虫变态过程中幼虫组织的降解和成虫器官的形成中起着至关重要的作用，伴随着预蛹-蛹-成虫的转变。然而，E93 调节的分子机制还知之甚少。在这项研究中，我们发现果蝇翅膀中 E93 基因的特异性敲低会破坏翅膀的发育。分析果蝇翅膀中 E93 的 ChIP-seq 信号表明，同源异形盒基因 decapentaplegic（Dpp）是 E93 的一个潜在下游靶标。ChIP-PCR 分析和双荧光素酶报告基因 assay 证实 E93 可以结合 Dpp 启动子并增强其活性。此外，在果蝇 S2 细胞中过表达 E93 会上调 Dpp 及其信号通路中其他成分的表达，但在翅膀中敲低 E93 后表达量下降。此外，Dpp 信号通路的损伤模拟了 E93 敲低对翅膀发育的缺陷。总之，我们的研究结果表明，E93 通过调节 Dpp 信号通路来调节果蝇变态过程中的翅膀发育。
