Department of Entomology, China Agricultural University, 100193 Beijing, China.
Development. 2013 Jul;140(14):2917-22. doi: 10.1242/dev.094300.
The control of organ growth is a fundamental aspect of animal development but remains poorly understood. The morphogen Dpp has long been considered as a general promoter of cell proliferation during Drosophila wing development. It is an ongoing debate whether the Dpp gradient is required for the uniform cell proliferation observed in the wing imaginal disc. Here, we investigated how the Dpp signaling pathway regulates proliferation during wing development. By systematic manipulation of Dpp signaling we observed that it controls proliferation in a region-specific manner: Dpp, via omb, promoted proliferation in the lateral and repressed proliferation in the medial wing disc. Omb controlled the regional proliferation rate by oppositely regulating transcription of the microRNA gene bantam in medial versus lateral wing disc. However, neither the Dpp nor Omb gradient was essential for uniform proliferation along the anteroposterior axis.
器官生长的控制是动物发育的一个基本方面，但仍知之甚少。形态发生素 Dpp 长期以来一直被认为是果蝇翅膀发育过程中促进细胞增殖的通用因子。目前仍存在争议的是，Dpp 浓度梯度是否是观察到的翅膀成虫盘均匀细胞增殖所必需的。在这里，我们研究了 Dpp 信号通路如何在翅膀发育过程中调节增殖。通过对 Dpp 信号的系统操作，我们观察到它以特定于区域的方式控制增殖：Dpp 通过 omb 在侧翼促进增殖，在中侧抑制增殖。Omb 通过在中侧和侧翼翅膀盘的 bantam 微 RNA 基因的转录的相反调节来控制区域增殖率。然而，Dpp 和 Omb 浓度梯度都不是沿前后轴均匀增殖所必需的。
