Rodríguez P B, Fernández A O, Corral A, Balcerzyk M, Risco R
Departamento de Física Aplicada III. Escuela Superior de Ingenieros, Universidad de Sevilla.
Departamento de Tecnología Electrónica. Escuela Técnica Superior de Ingeniería Informática. Universidad de Sevilla.
Cryo Letters. 2019 Jan/Feb;40(1):45-50.
In cryopreservation of biological material, it is very important to precise control the perfusion of cryoprotectants (CPA) inside the sample. CPA concentration inside tissues and organs during the process was measured in a few studies. The simulation of the CPA perfusion inside the organ is still necessary to understand and optimize this complex process.
This study simulates the diffusion of MeSO and PBS in a rabbit liver.
The software COMSOL for computational fluid-dynamics has been used. A hypothermic perfusion is simulated where temperature and pressure at the entrance of the organ are constant.
The simulation shows that MeSO concentration increases within the porous medium until saturation. The variation of perfusion speed and pressure inside the organ is almost null with time.
Finite elements modelling shows that under hypothermic conditions it is possible a full and even loading of this organ with MeSO, keeping constant the perfusion parameters.
在生物材料的冷冻保存过程中，精确控制冷冻保护剂（CPA）在样品内部的灌注非常重要。一些研究测量了该过程中组织和器官内部的CPA浓度。为了理解和优化这个复杂的过程，对器官内部的CPA灌注进行模拟仍然是必要的。
本研究模拟了MeSO和PBS在兔肝脏中的扩散。
使用了用于计算流体动力学的COMSOL软件。模拟了低温灌注，其中器官入口处的温度和压力保持恒定。
模拟显示MeSO浓度在多孔介质内升高直至饱和。器官内部灌注速度和压力随时间的变化几乎为零。
有限元建模表明，在低温条件下，可以使该器官完全且均匀地加载MeSO，同时保持灌注参数恒定。
