Genome-wide identification and co-expression network analysis of nuclear factor-Y in barley revealed potential functions in salt stress.

Nuclear factor-Ys (-) were previously shown to have important regulatory impacts in different developmental and physiological process. However, in barley the function of the NF-Y genes at system levels is not well known. To identify barley -, Arabidopsis and wheat NF-Y protein sequences were retrieved and the BLAST program along with the hidden Markov model were used. Multiple sequence alignments of identified - were constructed using ClustalW. Expression patterns of the - at different physiological and developmental conditions were also surveyed based on microarray datasets in public databases and subsequently co-expression network were constructed. Validation of in silico expression analysis was performed by real-time qPCR under salt stress condition. In total, 23 barley - (8 -, 11 - and 4 -) were identified. Based on the sequence homology, the subunits of the - complex were divided into three to five groups. Structural analysis highlighted the conserved domains of -, - and -. Co-expression network analysis indicated the potential functions of -s in photosynthesis, starch biosynthesis and osmotic stress tolerance. The results of qRT-PCR also confirmed the - roles in adaptation responses of barley to salt stress. We identified some potential candidate genes which could be used for improvements of cereals tolerance to salinity stress.