DC.提取物对活化肝星状细胞的治疗作用。
Therapeutic effect of DC. extracts on activated hepatic stellate cells.
作者信息
Basso Bruno de Souza, de Mesquita Fernanda Cristina, Dias Henrique Bregolin, Krause Gabriele Catyana, Scherer Matheus, Santarém Eliane Romanato, de Oliveira Jarbas Rodrigues
机构信息
PUCRS, Escola de Ciências, Laboratório de Pesquisa em Biofísica Celular e Inflamação, Porto Alegre, Brazil.
Graduate Program in Neuroengineering, Edmond and Lily Safra International Institute of Neuroscience, Santos Dumont Institute, Macaiba, Brazil.
出版信息
EXCLI J. 2019 Feb 18;18:91-105. eCollection 2019.
The therapeutic potential of DC. extracts was evaluated through its cytotoxic and antiproliferative effect and their phenotypic reversion property in activated hepatic stellate cells (HSCs). is distributed in Brazil (southeastern and south regions) and used for diuretic effect in folk medicine. Four fractions were obtained from the fractionation of the methanolic extract. Fractions III and IV decreased cell proliferation without increasing cell necrosis markers levels and induced cell cycle arrest in G1 phase. Fraction III induced phenotypic reversion through PPAR-γ activation pathway, while fraction IV did not alter PPAR-α/γ expression levels, suggesting that there is an independent PPAR-α/γ pathway involved. Hydroxybenzoic, chlorogenic and coumaric acids were identified. Fractions III and IV showed antiproliferative effect and ability to induce reversion of activated phenotype of HSCs.
通过其对活化肝星状细胞（HSCs）的细胞毒性和抗增殖作用及其表型逆转特性，评估了DC提取物的治疗潜力。DC分布于巴西（东南部和南部地区），在民间医学中用于利尿作用。从甲醇提取物的分馏中获得了四个馏分。馏分III和IV降低了细胞增殖，而没有增加细胞坏死标志物水平，并诱导细胞周期停滞在G1期。馏分III通过PPAR-γ激活途径诱导表型逆转，而馏分IV未改变PPAR-α/γ表达水平，表明存在独立的PPAR-α/γ途径。鉴定出了羟基苯甲酸、绿原酸和香豆酸。馏分III和IV显示出抗增殖作用以及诱导活化的HSCs表型逆转的能力。
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