Pereira Cristiane A, Costa Anna Carolina B Pereira, Liporoni Priscila Christiane S, Rego Marcos A, Jorge Antonio Olavo C
Unesp - Univ Estadual Paulista, School of Dentistry, Institute of Science and Technology, Department of Biosciences and Oral Diagnosis, Francisco José Longo 777, São Dimas, São José dos Campos, CEP: 12245-000 SP, Brazil.
Unitau - Univ de Taubaté, School of Dentistry, Department of Restorative Dentistry, Expedicionário Ernesto Pereira 110, Centro, Taubaté, CEP: 12020-330 SP, Brazil.
J Infect Public Health. 2016 May-Jun;9(3):324-30. doi: 10.1016/j.jiph.2015.10.012. Epub 2015 Nov 21.
Streptococcus mutans is an important cariogenic microorganism, and alternative methods for its elimination are required. Different concentrations of Baccharis dracunculifolia essential oil (EO) were tested to determine its minimal inhibitory concentration (MIC) in planktonic cultures, and this concentration was used in S. mutans biofilms. Additionally, we assessed the effect of a 0.12% chlorhexidine (CHX) and saline solution in S. mutans biofilms. The biofilms were grown in discs of composite resin for 48h and exposed to B. dracunculifolia, CHX or saline solution for 5min. The viability of the biofilms was determined by counting the colony-forming units per milliliter (CFU/ml) in agar, which was statistically significant (P<0.05). The MIC of the B. dracunculifolia EO to planktonic growth of S. mutans was 6%. In biofilms of S. mutans clinical isolates, B. dracunculifolia EO (6%) and CHX resulted in reductions of 53.3-91.1% and 79.1-96.6%, respectively. For the biofilm formed by the S. mutans reference strain, the reductions achieved with B. dracunculifolia EO and CHX were, respectively, 39.3% and 88.1%. It was concluded that B. dracunculifolia EO showed antibacterial activity and was able to control this oral microorganism, which otherwise causes dental caries.
变形链球菌是一种重要的致龋微生物，因此需要有替代方法来消除它。我们测试了不同浓度的巴西龙芽草精油（EO），以确定其在浮游培养物中的最低抑菌浓度（MIC），并将该浓度用于变形链球菌生物膜。此外，我们评估了0.12%的氯己定（CHX）和盐溶液对变形链球菌生物膜的影响。生物膜在复合树脂圆盘上生长48小时，然后暴露于巴西龙芽草、CHX或盐溶液中5分钟。通过计算琼脂中每毫升的菌落形成单位（CFU/ml）来确定生物膜的活力，结果具有统计学意义（P<0.05）。巴西龙芽草精油对变形链球菌浮游生长的MIC为6%。在变形链球菌临床分离株的生物膜中，巴西龙芽草精油（6%）和CHX分别使菌落形成单位减少了53.3 - 91.1%和79.1 - 96.6%。对于由变形链球菌参考菌株形成的生物膜，巴西龙芽草精油和CHX分别使菌落形成单位减少了39.3%和88.1%。得出的结论是，巴西龙芽草精油具有抗菌活性，能够控制这种会导致龋齿的口腔微生物。
