姜黄素与卵磷脂复合物通过抗炎机制对白细胞介素-1β刺激的人软骨细胞的软骨保护作用。
Chondroprotective effect of curcumin and lecithin complex in human chondrocytes stimulated by IL-1β via an anti-inflammatory mechanism.
作者信息
Kim Leeseon, Kim Ji Yeon
机构信息
Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul, 01811 Korea.
出版信息
Food Sci Biotechnol. 2018 Sep 18;28(2):547-553. doi: 10.1007/s10068-018-0470-6. eCollection 2019 Apr.
Abstract
A complex of curcumin and lecithin developed to improve the solubility of curcumin, enhanced its chondroprotective effect via an anti-inflammatory mechanism. In macrophage, proinflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, prostaglandin E2 (PGE2), and nitric oxide (NO) were quantified. In addition, the activity of nuclear factor (NF)-κB was examined. With chondrocytes, inflammatory mediators were assessed by measuring the secretion levels of IL-6, IL-8, and PGE2, also the mRNA expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Metalloproteinases (MMPs), tissue inhibitor of metalloprotease (TIMP)-1, type II collagen (COL2), proteoglycan (PG), and hyaluronic acid (HA) were measured with respect to the articulation surface. The complex promoted the anti-inflammatory effect by the inhibition of inflammatory mediators. In addition, mRNA expression levels ameliorated. Furthermore, it was effective in decreasing extracellular secretion of polypeptides, also corresponding intracellular MMPs and TIMP-1. In conclusion, the complex may be developed as a functional supplement to maintain articulation health.
摘要
一种为提高姜黄素溶解度而研发的姜黄素与卵磷脂复合物，通过抗炎机制增强了其软骨保护作用。在巨噬细胞中，对促炎细胞因子进行了定量分析，如肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β、IL-6、前列腺素E2（PGE2）和一氧化氮（NO）。此外，还检测了核因子（NF）-κB的活性。对于软骨细胞，通过测量IL-6、IL-8和PGE2的分泌水平，以及环氧化酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）的mRNA表达来评估炎症介质。针对关节表面测量了金属蛋白酶（MMPs）、金属蛋白酶组织抑制剂（TIMP）-1、II型胶原蛋白（COL2）、蛋白聚糖（PG）和透明质酸（HA）。该复合物通过抑制炎症介质促进了抗炎作用。此外，mRNA表达水平得到改善。此外，它在减少多肽的细胞外分泌以及相应的细胞内MMPs和TIMP-1方面有效。总之，该复合物可开发成为一种维持关节健康的功能性补充剂。
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