Kim Leeseon, Kim Ji Yeon
Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul, 01811 Korea.
Food Sci Biotechnol. 2018 Sep 18;28(2):547-553. doi: 10.1007/s10068-018-0470-6. eCollection 2019 Apr.
A complex of curcumin and lecithin developed to improve the solubility of curcumin, enhanced its chondroprotective effect via an anti-inflammatory mechanism. In macrophage, proinflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, prostaglandin E2 (PGE2), and nitric oxide (NO) were quantified. In addition, the activity of nuclear factor (NF)-κB was examined. With chondrocytes, inflammatory mediators were assessed by measuring the secretion levels of IL-6, IL-8, and PGE2, also the mRNA expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Metalloproteinases (MMPs), tissue inhibitor of metalloprotease (TIMP)-1, type II collagen (COL2), proteoglycan (PG), and hyaluronic acid (HA) were measured with respect to the articulation surface. The complex promoted the anti-inflammatory effect by the inhibition of inflammatory mediators. In addition, mRNA expression levels ameliorated. Furthermore, it was effective in decreasing extracellular secretion of polypeptides, also corresponding intracellular MMPs and TIMP-1. In conclusion, the complex may be developed as a functional supplement to maintain articulation health.
一种为提高姜黄素溶解度而研发的姜黄素与卵磷脂复合物，通过抗炎机制增强了其软骨保护作用。在巨噬细胞中，对促炎细胞因子进行了定量分析，如肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β、IL-6、前列腺素E2（PGE2）和一氧化氮（NO）。此外，还检测了核因子（NF）-κB的活性。对于软骨细胞，通过测量IL-6、IL-8和PGE2的分泌水平，以及环氧化酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）的mRNA表达来评估炎症介质。针对关节表面测量了金属蛋白酶（MMPs）、金属蛋白酶组织抑制剂（TIMP）-1、II型胶原蛋白（COL2）、蛋白聚糖（PG）和透明质酸（HA）。该复合物通过抑制炎症介质促进了抗炎作用。此外，mRNA表达水平得到改善。此外，它在减少多肽的细胞外分泌以及相应的细胞内MMPs和TIMP-1方面有效。总之，该复合物可开发成为一种维持关节健康的功能性补充剂。
