miRNAs 的差异表达及其在瘢痕疙瘩中 miR-194-3p 作用机制的初步研究。
The Differential Expression of miRNAs and a Preliminary Study on the Mechanism of miR-194-3p in Keloids.
机构信息
Department of Plastic and Reconstructive Surgery, The General Hospital of North Theater, PLA, Shenyang, China.
The General Hospital of Shenyang Military Region, Postgraduate Training Base of Jinzhou Medical University, Shenyang, China.
出版信息
Biomed Res Int. 2019 Mar 7;2019:8214923. doi: 10.1155/2019/8214923. eCollection 2019.
The aim of this study was to detect abnormally expressed microRNA (miRNA) in keloids and to study their functions. The differential expression of miRNAs in keloids and normal tissue was detected by gene microarray. MiRNA expression was verified by real-time PCR. A luciferase reporter gene assay, western blot, and real-time PCR were used to detect the effect of miR-194-3p on RUNX2. An MTT assay and a transwell assay were used to detect the effect of miR-194-3p in both primary cultured fibroblasts and HKF cells. Related proteins were analysed by western blot and real-time PCR. The expression of miR-194-3p was lower in keloids, and MiR-194-3p was shown to target RUNX2 directly. MiR-194-3p inhibited the proliferation and migration of fibroblasts through the inhibition of CDK4 and MMP2. MiR-194-3p and RUNX2 may become new targets for the prevention and treatment of keloids.
本研究旨在检测瘢痕疙瘩中异常表达的 microRNA（miRNA），并研究其功能。通过基因芯片检测瘢痕疙瘩和正常组织中 miRNA 的差异表达，并用实时 PCR 进行验证。通过荧光素酶报告基因检测、western blot 和实时 PCR 检测 miR-194-3p 对 RUNX2 的影响。用 MTT 检测和 Transwell 检测 miR-194-3p 对原代培养成纤维细胞和 HKF 细胞的影响。通过 western blot 和实时 PCR 分析相关蛋白。miR-194-3p 在瘢痕疙瘩中表达降低，并且 MiR-194-3p 被证明可以直接靶向 RUNX2。miR-194-3p 通过抑制 CDK4 和 MMP2 抑制成纤维细胞的增殖和迁移。miR-194-3p 和 RUNX2 可能成为预防和治疗瘢痕疙瘩的新靶点。
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