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[林蛙胚胎器官外植体中造血细胞的分化]

[Differentiation of hematopoietic cells in explants of embryonal organs of Rana temporaria L].

作者信息

Latsis R V, Saraeva N Iu

出版信息

Ontogenez. 1978;9(5):524-7.

PMID:309575
Abstract

The potencies of isolated embryonic hemopoietic organs (pronephros and liver) of Rana temporaria L. to the formation of the foci of hemopoiesis were studied. The pronephros and liver rudiments were explanted at the early developmental stages (late neurula and early tail bud) and cultivated in vivo in the diffusion chambers. The blast hemopoietic elements and differentiated blood cells are found in the explants within 7 to 10 days of cultivation. A suggestion is put forward that the differentiation of hemopoietic cells in the embryonic hemopoietic organs proceeds from the local cells-precursors.

摘要

研究了林蛙胚胎期分离的造血器官(前肾和肝脏)形成造血灶的能力。在前肾和肝脏原基处于发育早期阶段(神经胚晚期和尾芽早期)时将其移出,并在扩散小室中进行体内培养。培养7至10天内在移出物中发现了胚性造血成分和分化的血细胞。有人提出胚胎造血器官中造血细胞的分化是从局部细胞前体开始的。

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