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来自印度东海岸中部维沙卡帕特南的重要商业鲷科鱼类(鲈形目;笛鲷科)的DNA条形码分析

DNA barcoding of commercially important snapper species (Lutjaniformes; Lutjanidae; ) from Visakhapatnam, Central Eastern coast of India.

作者信息

Velamala Govinda Rao, Naranji Muddula Krishna, Kondamudi Ramesh Babu, Netto-Ferreira Andre Luiz

机构信息

a Department of Marine Living Resources , College of Science and Technology , Visakhapatnam , India.

b Laboratory of Ichthyology, Department of Zoology , Institute of Biosciences, Universidade Federal Do Rio Grande Do Sul , Porto Alegre , RS , Brazil.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2019 May;30(4):585-591. doi: 10.1080/24701394.2018.1551387. Epub 2019 Apr 7.

Abstract

Snappers are commercially important fishes in Indian waters, currently belonging to the order Lutjaniformes, family Lutjanidae. Generally, recognizing species of is a challenging task not only because of overlapping morphological characters, such as shapes, size groups, or colour patterns, but also based on the definition of the species concept or the definition of the threshold for speciation. In India there has not been any updated and accurate study of the genus so far. Besides, identification of the group based on ecological aspects and DNA barcoding tools were confined to limited laboratories. In the present study, ten species of snappers were identified from samples obtained from the major fish landing centres in the Visakhapatnam, Central Eastern coast of India. Snapper species were identified using COI (Cytochrome oxidase I) sequences for DNA barcoding. The validity of the conjecture species-level taxonomy based on COI is assisted with high equivalence search (98-100%) both in BOLD and BLAST, well-distributed genetic distance values.

摘要

笛鲷是印度海域具有重要商业价值的鱼类,目前隶属于笛鲷目笛鲷科。一般来说,识别笛鲷物种是一项具有挑战性的任务,这不仅是因为其形态特征存在重叠,如形状、大小组别或颜色模式,还基于物种概念的定义或物种形成阈值的定义。到目前为止,印度尚未对该属进行任何更新且准确的研究。此外,基于生态方面和DNA条形码工具对该类群的鉴定仅限于少数实验室。在本研究中,从印度东海岸中部维沙卡帕特南的主要鱼类上岸中心采集的样本中鉴定出了10种笛鲷。利用细胞色素氧化酶I(COI)序列进行DNA条形码分析来鉴定笛鲷物种。基于COI的推测物种水平分类法的有效性在生命条形码数据系统(BOLD)和基本局部比对搜索工具(BLAST)中均通过高相似度搜索(98 - 100%)以及分布良好的遗传距离值得到了佐证。

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