Laboratory of Marine Biology and Ecology, Third Institute of Oceanography State Oceanic Administration, Xiamen, Fujian, China.
PLoS One. 2018 Jun 1;13(6):e0198109. doi: 10.1371/journal.pone.0198109. eCollection 2018.
DNA barcoding based on a fragment of the cytochrome c oxidase subunit I (COI) gene in the mitochondrial genome is widely applied in species identification and biodiversity studies. The aim of this study was to establish a comprehensive barcoding reference database of fishes in the Taiwan Strait and evaluate the applicability of using the COI gene for the identification of fish at the species level. A total of 284 mitochondrial COI barcode sequences were obtained from 85 genera, 38 families and 12 orders of fishes. The mean length of the sequences was 655 base pairs. The average Kimura two parameter (K2P) distances within species, genera, families, orders and classes were 0.21%, 6.50%, 23.70% and 25.60%, respectively. The mean interspecific distance was 31-fold higher than the mean intraspecific distance. The K2P neighbor-joining trees based on the sequence generally clustered species in accordance with their taxonomic classifications. High efficiency of species identification was demonstrated in the present study by DNA barcoding, and we conclude that COI sequencing can be used to identify fish species.
基于线粒体基因组中细胞色素 c 氧化酶亚基 I（COI）基因片段的 DNA 条形码广泛应用于物种鉴定和生物多样性研究。本研究旨在建立台湾海峡鱼类综合条形码参考数据库，并评估 COI 基因在物种水平上识别鱼类的适用性。从 85 属、38 科和 12 目鱼类中获得了 284 条线粒体 COI 条形码序列。序列的平均长度为 655 个碱基对。种内、属内、科内、目内和类内的平均 Kimura 双参数（K2P）距离分别为 0.21%、6.50%、23.70%和 25.60%。种间平均距离比种内平均距离高 31 倍。基于序列的 K2P 邻接法树通常按照分类学分类聚类物种。本研究通过 DNA 条形码证明了高效的物种鉴定效率，我们得出结论，COI 测序可用于识别鱼类物种。
