一种新型血小板活化因子（PAF）拮抗剂（BN 52021）对豚鼠肺脏抗原诱导的前列腺素和血栓素形成的抑制作用。

Inhibitory effects of a novel platelet activating factor (PAF) antagonist (BN 52021) on antigen-induced prostaglandin and thromboxane formation by the guinea pig lung.

作者信息

Harczy M, Maclouf J, Pradelles P, Braquet P, Borgeat P, Sirois P

出版信息

Pharmacol Res Commun. 1986 Aug;18 Suppl:111-7. doi: 10.1016/0031-6989(86)90043-3.

Abstract

The effects of a new Platelet-Activating Factor (PAF-acether) antagonist (BN 52021) extracted from Ginkgo biloba leaves have been studied on the release of metabolites of arachidonic acid in IgG-dependent guinea pig pulmonary anaphylaxis in vitro. Prostaglandin E2 (PGE2) and thromboxane B2 (TxB2) were assayed using a novel ELISA technique. The release of PGE2 and TxB2 from anaphylactic lungs reached a maximum 4-5 min following the antigen challenge (about 3.2 and 31.0 ng/ml respectively) and decayed slowly during the following 25 min. BN 52021 (1, 3 and 30 micrograms/ml) produced dose-dependent decreases of the release of PGE2 and TxB2. These results suggest that there are some interactions between the release of icosanoids and PAF-acether in anaphylaxis.

摘要

对从银杏叶中提取的一种新型血小板活化因子（PAF-乙醚）拮抗剂（BN 52021）在体外IgG依赖性豚鼠肺过敏反应中花生四烯酸代谢产物释放方面的作用进行了研究。使用一种新型酶联免疫吸附测定（ELISA）技术测定前列腺素E2（PGE2）和血栓素B2（TxB2）。抗原攻击后4-5分钟，过敏肺中PGE2和TxB2的释放达到最大值（分别约为3.2和31.0纳克/毫升），并在随后的25分钟内缓慢下降。BN 52021（1、3和30微克/毫升）使PGE2和TxB2的释放呈剂量依赖性降低。这些结果表明，在过敏反应中，类二十烷酸的释放与PAF-乙醚之间存在一些相互作用。