Department of Public Health Sciences, Pennsylvania State University College of Medicine, Hershey, Pennsylvania.
Smith Institute for Urology, Zucker School of Medicine at Hofstra/Northwell, New Hyde Park, New York.
Prostate. 2019 Jun;79(9):961-968. doi: 10.1002/pros.23803. Epub 2019 Apr 8.
MicroRNAs (miRNAs or miR-) have been linked to factors associated with aggressive prostate cancer such as biochemical recurrence and metastasis. We investigated whether circulating miRNAs in plasma could be used as diagnostic biomarkers for more aggressive prostate cancer at prostate biopsy.
Men, aged 40 years and above, newly diagnosed with prostate cancer were categorized into two risk groups, low-grade (Gleason score, 6 or 7 [3 + 4] and serum prostate-specific antigen [PSA], <20 ng/mL) and high-grade (Gleason score, ≥7 (4 + 3) and serum PSA, ≥20 ng/mL) prostate cancers. The limma R package was used to compare the expression of miRNAs in plasma between the two risk groups, adjusting for age.
There were 66 men, aged 46-86 years, included: 40 men with low-grade and 26 men with high-grade prostate cancers. There were lower expressions of miR-28, miR-100, miR-942, and miR-28-3p, and higher expressions of miR-708, miR-1298, miR-886-3p, miR-374, miR-376c, miR-202, miR-128a, and miR-185 in high-grade compared to low-grade prostate cancer cases at biopsy, after adjusting for age (P < 0.05). These differences were no longer statistically significant after adjusting the P values for multiple comparisons.
There was no circulating miRNA associated with high-grade prostate cancer at biopsy after adjusting for age and multiple comparisons. Nevertheless, relationships between these circulating miRNAs and high-grade prostate cancer were observed, which suggest them as promising prostate cancer biomarkers. Further investigation in a larger cohort may provide insight into their diagnostic potential for aggressive prostate cancer.
微小 RNA（miRNA 或 miR-）与前列腺癌的侵袭性相关因素有关，如生化复发和转移。我们研究了循环血浆 miRNA 是否可作为前列腺活检中更具侵袭性前列腺癌的诊断生物标志物。
年龄在 40 岁及以上、新诊断为前列腺癌的男性被分为两个风险组：低级别（Gleason 评分 6 或 7 [3+4]和血清前列腺特异性抗原 [PSA]，<20ng/mL）和高级别（Gleason 评分≥7（4+3）和血清 PSA，≥20ng/mL）前列腺癌。使用 limma R 包比较两组风险的血浆中 miRNA 的表达，调整年龄因素。
共有 66 名年龄 46-86 岁的男性，包括 40 名低级别前列腺癌患者和 26 名高级别前列腺癌患者。高级别前列腺癌与低级别前列腺癌相比，miR-28、miR-100、miR-942 和 miR-28-3p 的表达水平较低，miR-708、miR-1298、miR-886-3p、miR-374、miR-376c、miR-202、miR-128a 和 miR-185 的表达水平较高，经年龄调整（P<0.05）。在调整多重比较的 P 值后，这些差异不再具有统计学意义。
经年龄和多重比较调整后，活检中没有与高级别前列腺癌相关的循环 miRNA。然而，观察到这些循环 miRNA 与高级别前列腺癌之间存在关系，提示它们作为有前途的前列腺癌生物标志物。在更大的队列中进一步研究可能会深入了解它们对侵袭性前列腺癌的诊断潜力。
