Confer A W, Hall S M, Espe B H
Am J Vet Res. 1985 Dec;46(12):2440-3.
Two experiments were done on 57 steers. These cattle were allotted to 8 groups (4 groups/experiment) and vaccinated with 1 to 3 X 10(9) colony-forming units of Brucella abortus strain 19. Cattle in 3 of the 4 groups/experiment were given 6 mg of levamisole/kg, subcutaneously, either at the time of vaccination (day 0), 7 days later, or at both times. Serum antibody titers to B abortus were measured sequentially for 28 days in experiment 1 and for 56 days in experiment 2, using the card test, Rivanol test, complement-fixation test, fluorometric immunoassay, and an enzyme-linked immunosorbent assay. In general, the highest mean antibody titers, as determined by all serologic tests, occurred in steers treated with levamisole at 7 days after vaccination or in those treated at the time of vaccination and 7 days later. By the card test on day 56, there was a significantly (P less than 0.05) greater number of seropositive cattle among those given levamisole 7 days after seropositive cattle among those given strain 19 alone. Simultaneous administration of strain 19 and levamisole did not alter antibody responses to B abortus.
对57头阉牛进行了两项实验。这些牛被分成8组（每组实验4组），并用1至3×10⁹个流产布鲁氏菌19菌株的菌落形成单位进行接种。在每组实验的4组中，有3组的牛在接种时（第0天）、7天后或两次均皮下注射6毫克/千克的左旋咪唑。在实验1中连续28天、在实验2中连续56天，使用平板试验、瑞凡诺试验、补体结合试验、荧光免疫测定法和酶联免疫吸附测定法依次测量针对流产布鲁氏菌的血清抗体滴度。总体而言，通过所有血清学检测确定的最高平均抗体滴度出现在接种后7天接受左旋咪唑治疗的阉牛中，或出现在接种时和7天后接受治疗的阉牛中。通过第56天的平板试验，在接种后7天给予左旋咪唑的牛中，血清阳性牛的数量显著（P<0.05）多于仅接种19菌株的牛中的血清阳性牛。同时给予19菌株和左旋咪唑并未改变对流产布鲁氏菌的抗体反应。
