Paovalo C, Chulasiri M U
Department of Pharmaceutical Chemistry and Department of Microbiology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
J Food Prot. 1986 Jan;49(1):12-13. doi: 10.4315/0362-028X-49.1.12.
Further to a previous report (Food Chem. Toxicol. 22:109-112, 1984) describing the presence of mutagens in a chloroform extract of Ceylon cinnamon ( Cinnamomum zeylanicum Nees), we report here our findings on the mutagenicity of silica gel column chromatographic fractions from this extract. Mutagenicity was evaluated by the rec assay using Bacillus subtillis strains H17 (rec ) and M45 (rec). Fractions collected after elution with chloroform, chloroform-ethanol (98:2) and (95:5) exhibited mutagenicity, while those obtained by ellution with chloroform-ethanol (85:15), (75:25) and ethanol showed no such activity.
继之前一份报告（《食品化学与毒理学》22:109 - 112, 1984）描述锡兰肉桂（Cinnamomum zeylanicum Nees）氯仿提取物中存在诱变剂之后，我们在此报告该提取物硅胶柱色谱馏分的致突变性研究结果。采用枯草芽孢杆菌菌株H17（rec⁻）和M45（rec⁻）通过rec测定法评估致突变性。用氯仿、氯仿 - 乙醇（98:2）和（95:5）洗脱后收集的馏分具有致突变性，而用氯仿 - 乙醇（85:15）、（75:25）和乙醇洗脱得到的馏分则无此活性。
