Ungsurungsie M, Paovalo C, Noonai A
Food Chem Toxicol. 1984 Feb;22(2):109-12. doi: 10.1016/0278-6915(84)90089-9.
The extraction of about 1.9 kg of Ceylon cinnamon (Cinnamomum zeylanicum Nees) with 10 litres each of petroleum ether, chloroform and ethanol in a Soxhlet apparatus produced extracts weighing 76, 28 and 270 g respectively for the three solvents. In the preliminary test the ethanol extract showed no mutagenic activity. However, both the petroleum ether and the chloroform extracts showed mutagenicity when tested in the rec assay using Bacillus subtilis strains H17 (rec+) and M45 (rec-). When these extracts were studied quantitatively by the liquid and spore rec-assay methods, the minimum inhibitory concentrations of the extracts against strain H17 were higher than those against strain M45. However, in the presence of the liver S-9 mix, the minimum inhibitory concentrations of the petroleum ether and chloroform extracts against both strains of B. subtilis were equal, indicating that the mutagenicity of the extracts had been inactivated.
在索氏提取器中，分别用10升石油醚、氯仿和乙醇对约1.9千克锡兰肉桂（Cinnamomum zeylanicum Nees）进行提取，三种溶剂得到的提取物重量分别为76克、28克和270克。在初步试验中，乙醇提取物未显示出诱变活性。然而，在使用枯草芽孢杆菌菌株H17（rec +）和M45（rec -）的rec试验中测试时，石油醚提取物和氯仿提取物均显示出诱变性。当通过液体和孢子rec试验方法对这些提取物进行定量研究时，提取物对菌株H17的最低抑制浓度高于对菌株M45的最低抑制浓度。然而，在存在肝脏S - 9混合物的情况下，石油醚提取物和氯仿提取物对两种枯草芽孢杆菌菌株的最低抑制浓度相等，表明提取物的诱变性已被灭活。
