抗3'-OH-T-2毒素抗体的制备与鉴定

Production and Characterization of Antibody Against 3'-OH-T-2 Toxin.

作者信息

Wei Ru-Dong, Bischoff William, Chu Fun Sun

机构信息

Food Research Institute and Department of Food Microbiology and Toxicology, University of Wisconsin, 1925 Willow Drive, Madison, Wisconsin 53706.

出版信息

J Food Prot. 1986 Apr;49(4):267-271. doi: 10.4315/0362-028X-49.4.267.

DOI:10.4315/0362-028X-49.4.267

PMID:30959657

Abstract

Antibody raised against T-2 toxin cross-reacted poorly with 3'-OH-T-2 toxin. A new immunogen was prepared by conjugation of hemisuccinate (HS) of 3'-OH-T-2 toxin to bovine serum albumin (BSA). Antibodies against 3'-OH-T-2 toxin were demonstrated by a radioimmunoassay 10 wk after immunization of rabbits with this new immunogen using tritiated 3'-OH-T-2 toxin as the testing ligand. Highest titers (1:6,000) were obtained 17 wk after immunization and two booster injections. The antibodies had good cross-reactivity with T-2 toxin, acetyl-T-2 toxin and 3'-OH-acetyl-T-2 toxin. The relative cross-reactivity of this antibody with 3'-OH-T-2, acetyl-T-2, T-2, 3'-OHacetyl-T-2, 3'-OH-T-2-HS, T-2 isomer, HT-2 and 3'-OH-HT-2 was 1, 3, 4, 5, 15, 30, 45 and 175, respectively. No crossreaction was found when 3'-OH-T-2 triol, T-2-triol, T-2-tetraol, DAS and DON at a concentration of 1 μg per assay was tested. The detection limit for 3'-OH-T-2 toxin by the RIA was about 0.1 ng per assay.

摘要

针对T-2毒素产生的抗体与3'-OH-T-2毒素的交叉反应较弱。通过将3'-OH-T-2毒素的半琥珀酸酯（HS）与牛血清白蛋白（BSA）偶联制备了一种新的免疫原。用这种新免疫原免疫兔子10周后，使用氚标记的3'-OH-T-2毒素作为检测配体，通过放射免疫测定法证明了针对3'-OH-T-2毒素的抗体。免疫和两次加强注射17周后获得了最高滴度（1:6,000）。这些抗体与T-2毒素、乙酰-T-2毒素和3'-OH-乙酰-T-2毒素具有良好的交叉反应性。该抗体与3'-OH-T-2、乙酰-T-2、T-2、3'-OH-乙酰-T-2、3'-OH-T-2-HS、T-2异构体、HT-2和3'-OH-HT-2的相对交叉反应性分别为1、3、4、5、15、30、45和175。当以每次测定1μg的浓度测试3'-OH-T-2三醇、T-2-三醇、T-2-四醇、DAS和DON时，未发现交叉反应。放射免疫测定法对3'-OH-T-2毒素的检测限约为每次测定0.1ng。