Lillehoj E B, Jacks T J, Calvert O H
ARS, USDA, Southern Regional Research Center, New Orleans, Louisiana 70179 and Department of Plant Pathology, University of Missouri, Columbia, Missouri 65211.
J Food Prot. 1986 Aug;49(8):623-626. doi: 10.4315/0362-028X-49.8.623.
A procedure was developed for efficient extraction of bright greenish-yellow (BGY) fluorescent material from ground corn ( Zea mays L.) samples and subsequent quantitative determination of fluorescence emission in a fluorometer. The technique was designed to be inexpensive, rapid and utilizable at remote locations for estimation of aflatoxin levels. A simple, in vitro method for production of the BGY-fluorescing material from kojic acid was developed to provide a reference standard. Water was identified as an effective solvent for extraction of the BGY-fluorescence from corn; fluorescence in aqueous extracts was stable at pH 6-7 for up to 22 h at room temperature. Samples with aflatoxin concentrations of 0 to 12,000 ppb yielded BGY-fluorescence emission units of 0 to 175. A correlation coefficient of r = 0.77 was determined for BGY-fluorescence vs total aflatoxin levels (B, B, G, G) and r = 0.88 for the fluorescence vs aflatoxin B, B. The results provided a basis for effective determination of BGY-fluorescence as an estimate of aflatoxin levels in corn.
开发了一种从磨碎的玉米（玉米属）样品中高效提取亮绿黄色（BGY）荧光物质并随后在荧光计中定量测定荧光发射的方法。该技术设计得成本低廉、快速且可在偏远地区用于估计黄曲霉毒素水平。开发了一种从曲酸生产BGY荧光物质的简单体外方法，以提供参考标准。已确定水是从玉米中提取BGY荧光的有效溶剂；水提取物中的荧光在室温下pH 6 - 7时稳定长达22小时。黄曲霉毒素浓度为0至12,000 ppb的样品产生的BGY荧光发射单位为0至175。BGY荧光与总黄曲霉毒素水平（B1、B2、G1、G2）的相关系数r = 0.77，荧光与黄曲霉毒素B1、B2的相关系数r = 0.88。这些结果为有效测定BGY荧光作为玉米中黄曲霉毒素水平的估计提供了依据。
