Cole Mary Townsend, Kilgen Marilyn B, Hackney Cameron R
Department of Food Science, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 and Department of Biology, Nicholls State University, Thibodaux, Louisiana 70803.
J Food Prot. 1986 Aug;49(8):592-595. doi: 10.4315/0362-028X-49.8.592.
Six techniques were evaluated for recovery of poliovirus from Louisiana oysters. The methods were compared for percent recovery rates, toxicity, ease of extraction, bacterial contamination, and final volume of oyster concentrate. Oyster samples were contaminated with 30-40 plaque forming units of Poliovirus type 1 and processed by six variations of adsorption-elution-precipitation and elution-precipitation methods. The method developed by Ellender et al. (Natural enterovirus and fecal coliform contamination of gulf coast oysters. J. Food Prot. 43:105-110) was judged to be the preferred method for gulf coast oysters.
对六种从路易斯安那州牡蛎中回收脊髓灰质炎病毒的技术进行了评估。比较了这些方法在回收率、毒性、提取难易程度、细菌污染以及牡蛎浓缩液最终体积方面的情况。用1型脊髓灰质炎病毒的30 - 40个蚀斑形成单位污染牡蛎样本，并通过吸附 - 洗脱 - 沉淀和洗脱 - 沉淀方法的六种变体进行处理。埃伦德等人开发的方法（墨西哥湾沿岸牡蛎的天然肠道病毒和粪大肠菌群污染。《食品保护杂志》43:105 - 110）被判定为墨西哥湾沿岸牡蛎的首选方法。
