Key Laboratory of Marine Drugs, Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China.
Molecules. 2019 Apr 7;24(7):1365. doi: 10.3390/molecules24071365.
Intestinal mucins constitute the major component of the mucus covering the epithelium of the gastrointestinal tract, thereby forming a barrier against microbial colonization. Rabbits are bred in large numbers worldwide, with little known about intestinal O-glycosylation despite this insight being crucial to the understanding of host-pathogen interactions. In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches. Thirty-three O-glycans were identified, and most of them were sulfated or sialylated glycans. It was worth noting that Neu5Gc-containing structures within sialylated O-glycans accounted for 91%, which were extremely different from that of other species including humans, mice, chickens, etc. Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin. These structural characterization reflected species diversity and may provide deeper insights into explaining the adaptability of hyla rabbit to the environment.
肠道粘蛋白构成了覆盖胃肠道上皮的粘液的主要成分，从而形成了抵御微生物定植的屏障。兔子在全球范围内大量繁殖，尽管了解肠道 O-糖基化对于理解宿主-病原体相互作用至关重要，但对此知之甚少。在本研究中，分离了一种主要的粘蛋白型糖肽（RIF6），并基于液相色谱-串联质谱（LC-MS/MS）结合生物信息学方法对 O-聚糖进行了广泛的表征。鉴定出 33 种 O-聚糖，其中大多数为硫酸化或唾液酸化聚糖。值得注意的是，唾液酸化 O-聚糖中含有 Neu5Gc 的结构占 91%，这与包括人类、小鼠、鸡等在内的其他物种极为不同。硫酸化聚糖占 58%，在兔肠粘蛋白中还检测到独特的二硫酸化和硫酸化-唾液酸化聚糖。这些结构特征反映了物种多样性，并可能为解释树蟾兔对环境的适应性提供更深入的见解。
