Kim Sujin, Shin Byung Ho, Yang Chungmo, Jeong Soohyun, Shim Jung Hee, Park Min Hee, Choy Young Bin, Heo Chan Yeong, Lee Kangwon
Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Korea.
Department of Biomedical Engineering, College of Medicine, Seoul National University, Seoul 03080, Korea.
Polymers (Basel). 2018 Jul 13;10(7):772. doi: 10.3390/polym10070772.
The number of breast reconstruction surgeries has been increasing due to the increase in mastectomies. Surgical implants (the standard polydimethylsiloxane (PDMS) implants) are widely used to reconstruct breast tissues, however, it can cause problems such as adverse immune reactions, fibrosis, rupture, and additional surgery. Hence, polymeric fillers have recently garnered increasing attention as strong alternatives for breast reconstruction materials. Polymeric fillers offer noninvasive methods of reconstruction, thereby reducing the possible adverse effects and simplifying the treatment. In this study, we synthesized a 2-hydroxylethylmethacrylate (HEMA) and acrylamide (Am) copolymer (Poly(HEMA-Am)) by redox polymerization to be used as a biocompatible filler material for breast reconstruction. The synthesized hydrogel swelled in phosphate buffered saline (PBS) shows an average modulus of 50 Pa, which is a characteristic similar to that of the standard dermal acrylamide filler. To investigate the biocompatibility and cytotoxicity of the Poly(HEMA-Am) hydrogel, we evaluated an in vitro cytotoxicity assay on human fibroblasts (hFBs) and human adipose-derived stem cells (hADSCs) with the hydrogel eluate, and confirmed a cell viability of over 80% of the cell viability with the Poly(HEMA-Am) hydrogel. These results suggest our polymeric hydrogel is a promising filler material in soft tissue augmentation including breast reconstruction.
由于乳房切除术数量的增加，乳房重建手术的数量一直在上升。手术植入物（标准的聚二甲基硅氧烷（PDMS）植入物）被广泛用于重建乳房组织，然而，它可能会引发诸如不良免疫反应、纤维化、破裂以及额外手术等问题。因此，聚合物填充剂作为乳房重建材料的有力替代品，近来受到了越来越多的关注。聚合物填充剂提供了非侵入性的重建方法，从而减少了可能的不良影响并简化了治疗过程。在本研究中，我们通过氧化还原聚合反应合成了甲基丙烯酸2-羟乙酯（HEMA）与丙烯酰胺（Am）的共聚物（聚（HEMA-Am）），用作乳房重建的生物相容性填充材料。在磷酸盐缓冲盐水（PBS）中膨胀的合成水凝胶显示出平均模量为50 Pa，这一特性与标准的真皮丙烯酰胺填充剂相似。为了研究聚（HEMA-Am）水凝胶的生物相容性和细胞毒性，我们用该水凝胶洗脱液对人成纤维细胞（hFBs）和人脂肪来源干细胞（hADSCs）进行了体外细胞毒性测定，并证实使用聚（HEMA-Am）水凝胶时细胞活力超过80%。这些结果表明，我们的聚合物水凝胶在包括乳房重建在内的软组织填充方面是一种有前景的填充材料。
