免疫分析与 FLOTAC 诊断犬贾第虫属感染的成本效果比较。

Comparative cost-effectiveness of immunoassays and FLOTAC for diagnosing Giardia spp. infection in dogs.

机构信息

Department of Veterinary Medicine and Animal Production, University of Naples Federico II, CREMOPAR, Campania Region, Eboli, Italy.

Department of Veterinary Medicine, Federal Rural University of Pernambuco, R. Manuel de Medeiros s/n, Dois Irmãos, Recife, PE, 52171-900, Brazil.

出版信息

Parasit Vectors. 2019 Apr 8;12(1):158. doi: 10.1186/s13071-019-3425-8.

DOI:10.1186/s13071-019-3425-8

PMID:30961639

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6454601/

Abstract

BACKGROUND

Giardia spp. is a protozoan pathogen and is the most common enteric parasite of domestic animals and humans. Assays for detecting infection in fecal samples using direct or indirect examinations are important tools for diagnosing the disease. The objective of the present study was to compare the cost-effectiveness of immunoassays and FLOTAC technique for diagnosing Giardia spp. infection in dogs.

RESULTS

Fecal samples from 80 positive stray dogs were tested for the presence of copro-antigens of Giardia spp. using the direct immunofluorescence assay (IFA), a rapid enzyme-linked immunosorbent assay (ELISA) and the FLOTAC double technique. All methods were performed in accordance with the instructions reported in the original description for each technique. The results showed that ELISA can be run in less time than IFA and almost at the same time of the FLOTAC technique. Among the tests used in this study, FLOTAC had the lowest cost per correct diagnosis, compared with immunoassays.

CONCLUSIONS

The results from this cost-effectiveness analysis, in combination with the sensitivity and specificity of the FLOTAC technique, suggest that the FLOTAC technique can be use in the routine diagnosis of Giardia spp. infection in dogs.

摘要

背景

贾第虫属是一种原生动物病原体，是家畜和人类最常见的肠道寄生虫。使用直接或间接检查检测粪便样本中感染的检测方法是诊断该疾病的重要工具。本研究的目的是比较免疫测定法和 FLOTAC 技术在诊断犬贾第虫属感染中的成本效益。

结果

使用直接免疫荧光法（IFA）、快速酶联免疫吸附试验（ELISA）和 FLOTAC 双重技术检测了 80 只阳性流浪犬粪便中贾第虫属的粪抗原。所有方法均按照每种技术原始描述中报告的说明进行操作。结果表明，ELISA 比 IFA 运行时间更短，几乎与 FLOTAC 技术同时进行。在本研究中使用的检测方法中，与免疫测定法相比，FLOTAC 的每次正确诊断的成本最低。

结论

本成本效益分析的结果，结合 FLOTAC 技术的敏感性和特异性，表明 FLOTAC 技术可用于犬贾第虫属感染的常规诊断。

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免疫分析与 FLOTAC 诊断犬贾第虫属感染的成本效果比较。

Comparative cost-effectiveness of immunoassays and FLOTAC for diagnosing Giardia spp. infection in dogs.

机构信息

Department of Veterinary Medicine and Animal Production, University of Naples Federico II, CREMOPAR, Campania Region, Eboli, Italy.

Department of Veterinary Medicine, Federal Rural University of Pernambuco, R. Manuel de Medeiros s/n, Dois Irmãos, Recife, PE, 52171-900, Brazil.

出版信息

Parasit Vectors. 2019 Apr 8;12(1):158. doi: 10.1186/s13071-019-3425-8.

DOI:10.1186/s13071-019-3425-8

PMID:30961639

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6454601/

Abstract

BACKGROUND

RESULTS

CONCLUSIONS

摘要

背景

结果

结论

本成本效益分析的结果，结合 FLOTAC 技术的敏感性和特异性，表明 FLOTAC 技术可用于犬贾第虫属感染的常规诊断。

免疫分析与 FLOTAC 诊断犬贾第虫属感染的成本效果比较。

Comparative cost-effectiveness of immunoassays and FLOTAC for diagnosing Giardia spp. infection in dogs.

机构信息

出版信息

BACKGROUND

RESULTS

CONCLUSIONS

背景

结果

结论

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本文引用的文献

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免疫分析与 FLOTAC 诊断犬贾第虫属感染的成本效果比较。

Comparative cost-effectiveness of immunoassays and FLOTAC for diagnosing Giardia spp. infection in dogs.

机构信息

出版信息

BACKGROUND

RESULTS

CONCLUSIONS

背景

结果

结论