In Vitro Evaluation of Ozonated Water Treatment on the Viability of Oocysts and Cysts from Water Buffaloes: A Proof-of-Concept Study.

The aim of this proof-of-concept study was to evaluate the in vitro effects of ozonated water treatment on the viability of oocysts and cysts isolated from naturally infected water buffaloes. oocysts were divided into seven groups of six replicates that were treated with ozonated water at three ozone concentrations (0.5, 1, and 2 mg/L) and two contact times (five and ten minutes), and one group (negative control) that was exposed to non-treated water. cysts were divided into nine groups of six replicates and were treated with ozonated water at four ozone concentrations (0.1, 0.3, 0.5, and 1 mg/L) and two contact times (one and two minutes), while one group (negative control) was exposed to non-treated water. The results of the ozonated water treatment gave a 33% inhibition of the sporulation of oocysts and rendered 96.3% of cysts non-viable, suggesting that ozonated water treatment could be a promising alternative sanitation technology to common conventional disinfectants for reducing intestinal protozoa infections in water buffaloes; though further in vitro and in vivo tests are needed.