School of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
School of Pharmaceutical Sciences (Shenzhen), Sun Yat-Sen University, Shenzhen 518107, China.
J Nanosci Nanotechnol. 2019 Sep 1;19(9):5496-5502. doi: 10.1166/jnn.2019.16592.
The Alisol A 24-acetate is an effective component of the Alismatis Rhizoma (AR) extract, which is often used in the treatment of hyperlipidemia. This study explored the effect and mechanism of the Alisol A 24-acetate from AR on lipid deposition in the liver of hyperlipidemic mice. After establishing hyperlipidemic mouse model (Model) by oral high-fat diet (HFD), the animals were treated with Alisol A 24-acetate for 4 weeks. The changes of blood lipid in mice were detected by ELISA. Hematoxylin and eosin (H&E) staining was used to evaluate the degree of liver lipid deposition in hyperlipidemic mice. Quantitative reverse transcriptase PCR (RT-qPCR) was used to detect the expression of ABCG1 and ABCA1 mRNA in the liver. The expression of ABCG1 and ABCA1 protein was detected by Western blotting (WB). After 4 weeks of high-fat diet, the levels of TC, TG, and LDL-C in the mouse were increased, and the HDL-C level was decreased. After treatment with Alisol A 24-acetate, the levels of TC, TG, and LDL-C in the blood of hyperlipidemic mouse were significantly reduced, and the level of HDL-C was increased. The results of H&E staining showed that the lipid deposition in the liver of hyperlipidemic mouse was improved after treatment with Alisol A 24-acetate. RT-qPCR and WB analysis documented that ABCG1 and ABCA1 mRNA and protein expression in hyperlipidemic mice were promoted after the Alisol A 24-acetate treatment. In conclusion, Alisol A 24-acetate effectively alleviates the liver lipid deposition in hyperlipidemic mice, and this effect is achieved mostly by promoting the expression of ABCG1 and ABCA1 at the mRNA and protein levels.
泽泻醇 A-24-醋酸酯是泽泻提取物（AR）中的一种有效成分，常用于治疗高血脂症。本研究探讨了泽泻醇 A-24-醋酸酯对高脂饮食诱导的高血脂症小鼠肝脏脂质沉积的作用及机制。通过口服高脂饮食（HFD）建立高血脂症小鼠模型（Model）后，用泽泻醇 A-24-醋酸酯处理 4 周。采用 ELISA 检测小鼠血脂变化，苏木精-伊红（H&E）染色评估高血脂小鼠肝脏脂质沉积程度，定量逆转录 PCR（RT-qPCR）检测肝脏 ABCG1 和 ABCA1mRNA 的表达，Western blot（WB）检测 ABCG1 和 ABCA1 蛋白的表达。经 4 周高脂饮食后，小鼠 TC、TG 和 LDL-C 水平升高，HDL-C 水平降低；经泽泻醇 A-24-醋酸酯处理后，高血脂小鼠血液中 TC、TG 和 LDL-C 水平显著降低，HDL-C 水平升高。H&E 染色结果显示，泽泻醇 A-24-醋酸酯处理后高血脂小鼠肝脏脂质沉积得到改善。RT-qPCR 和 WB 分析表明，泽泻醇 A-24-醋酸酯处理后高血脂小鼠 ABCG1 和 ABCA1mRNA 和蛋白表达增加。综上所述，泽泻醇 A-24-醋酸酯能有效减轻高血脂症小鼠肝脏脂质沉积，这种作用主要通过促进 ABCG1 和 ABCA1 的表达来实现。
