Department of Otolaryngology, Affiliated Hospital of Hebei University, Hebei, China.
Yonsei Med J. 2021 Oct;62(10):895-902. doi: 10.3349/ymj.2021.62.10.895.
Alisol A is a bioactive triterpenoid isolated from the Rhizoma Alismatis. Previous studies have shown that alisol A has anticancer potential. In this study, we explored the effect of alisol A on the growth of nasopharyngeal carcinoma (NPC) cells.
MTT assay, colony formation assay, flow cytometry, transwell assay, wound healing assay, and western blotting were used to assess cell viability, proliferation, cell cycle, migration, invasion, and protein expression, respectively, in vitro. AutoDock Vina and Discovery Studio software were used for molecular docking.
Alisol A inhibited the viability, proliferation, migration, and invasion of NPC cells. The molecular docking simulation assay confirmed that alisol A bound to YAP protein. In addition, alisol A promoted the phosphorylation of YAP and suppressed the expression of YAP in NPC cells.
Alisol A inhibited the proliferation, migration, and invasion of NPC cells by inhibiting the Hippo signaling pathway. Alisol A may be a candidate drug for NPC.
冬凌草甲素是从泽泻属植物中分离得到的一种生物活性三萜。先前的研究表明，冬凌草甲素有抗癌潜力。本研究探讨了冬凌草甲素对鼻咽癌细胞（NPC）生长的影响。
MTT 检测、集落形成实验、流式细胞术、Transwell 实验、划痕愈合实验和 Western blot 分别用于评估细胞活力、增殖、细胞周期、迁移、侵袭和蛋白表达。AutoDock Vina 和 Discovery Studio 软件用于分子对接。
冬凌草甲素抑制 NPC 细胞的活力、增殖、迁移和侵袭。分子对接模拟实验证实冬凌草甲素与 YAP 蛋白结合。此外，冬凌草甲素促进 NPC 细胞中 YAP 的磷酸化，并抑制 YAP 的表达。
冬凌草甲素通过抑制 Hippo 信号通路抑制 NPC 细胞的增殖、迁移和侵袭。冬凌草甲素可能是 NPC 的候选药物。
