Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, Guangdong 518033, China.
School of Applied Chemistry and Biotechnology, Shenzhen Polytechnic, Shenzhen, Guangdong 518055, China.
J Nanosci Nanotechnol. 2019 Sep 1;19(9):5989-5993. doi: 10.1166/jnn.2019.16501.
() is a major cause of urinary tract infections. Treatment of these infections with antibiotics is often not effective due to the acquisition of drug-resistance genes by the bacteria. This process is mediated by integrons which belong to bacterial mobile genetic elements. Therefore, the present study addressed the issue of the relation between antibiotic resistance and integron genes in isolated from patients affected by urinary tract infection. Multiplex PCR assay employed to detect the integrase gene demonstrated that out of 49 bacterial strains, 26 were carrying class 1 integron and there was no case of bacteria harboring class 2 or class 3 integrons. Correlation analysis documented that strains harboring class 1 integron exhibited higher resistance towards tobramycin. The variable region gene cassette contained combinations of four genes responsible for antibiotic resistance: , and , of which the latter conferred tobramycin resistance. Together, the collected data underscore the need for identification and analysis of integrons in -induced urinary infections.
() 是尿路感染的主要原因。由于细菌获得了耐药基因，这些感染的抗生素治疗往往效果不佳。这一过程是由整合子介导的，整合子属于细菌移动遗传元件。因此，本研究探讨了从尿路感染患者中分离出的 与整合子基因之间的关系。采用多重 PCR 检测方法检测 整合酶基因，结果表明，在 49 株细菌中，有 26 株携带 1 类整合子，没有 2 类或 3 类整合子的细菌。相关性分析表明，携带 1 类整合子的菌株对妥布霉素的耐药性更高。可变区基因盒包含四个对抗生素耐药的基因组合： 、 、和 ，其中后者赋予妥布霉素耐药性。综上所述，这些数据强调了在 - 引起的尿路感染中识别和分析整合子的必要性。
