Vidal-Gomez J
Scand J Immunol. 1978;8(4):323-31. doi: 10.1111/j.1365-3083.1978.tb00525.x.
Dinitrophenyl (DNP)-lysine-polymethylmethacrylate and DNP-cellulose conjugates do not irreversibly inactivate anti-DNP antigen-sensitive cells, regardless of the dose (up to 10 mg) or persistence of the stimulation (up to 2 weeks). Since these conjugates constitute pure hapten presentations, it is concluded that the pure hapten presentation to B lymphocyte does not irreversibly inactivate them. When murine spleen cells are cultured with Escherichia coli lipopolysaccharide (LPS) and (non-immunogenic) DNP-lysine-polymethylmethacrylate or (non-immunogenic) DNP-cellulose conjugates, an anti-DNP immune response occurs. However, replacement of DNP-lysine-polymethylmethacrylate with polymethylmethacrylate, or DNP-cellulose with cellulose, also results in a similar anti-DNP response. It is consequently concluded that the anti-DNP responses are entirely elicited by LPS, the hapten Dnp being inoperative. The anti-DNP response elicited by DNP-Ficoll is, upon exhaustive testing, carrier-dependent. This implies that the mechanism of DNP-Ficoll immunogenicity is not two cooperative signals passed on to B lymphocytes via the hapten DNP. These results argue against any two-signal model of B-lymphocyte activation.
二硝基苯基（DNP）-赖氨酸-聚甲基丙烯酸甲酯和DNP-纤维素缀合物不会不可逆地使抗DNP抗原敏感细胞失活，无论剂量（高达10毫克）或刺激持续时间（长达2周）如何。由于这些缀合物构成了纯半抗原呈现，因此得出结论，向B淋巴细胞呈现纯半抗原不会不可逆地使其失活。当用大肠杆菌脂多糖（LPS）和（非免疫原性的）DNP-赖氨酸-聚甲基丙烯酸甲酯或（非免疫原性的）DNP-纤维素缀合物培养小鼠脾细胞时，会发生抗DNP免疫反应。然而，用聚甲基丙烯酸甲酯替代DNP-赖氨酸-聚甲基丙烯酸甲酯，或用纤维素替代DNP-纤维素，也会导致类似的抗DNP反应。因此得出结论，抗DNP反应完全由LPS引发，半抗原Dnp不起作用。经全面测试，DNP-菲可引发的抗DNP反应依赖载体。这意味着DNP-菲可免疫原性的机制不是通过半抗原DNP传递给B淋巴细胞的两个协同信号。这些结果反对B淋巴细胞激活的任何双信号模型。
