Meidinger R G, Bentley M M
Biochem Genet. 1986 Oct;24(9-10):683-99. doi: 10.1007/BF00499002.
The aldox-2 locus in Drosophila melanogaster has been shown to affect differentially three molybdoenzymes, aldehyde oxidase, pyridoxal oxidase, and xanthine dehydrogenase. These effects are most obvious at times surrounding the pupal-adult boundary, when the normal organism accumulates large amounts of these enzymes in their active form. This locus has been more precisely mapped genetically to 2-82.9 +/- 2.1, with complete concordance between the effects of all recombinant chromosomes on all three enzymes. The cytogenetic location has also been determined to be between 52E and 54E8, with the likelihood that it lies within the region 54B1-54E8. The aldox-2 mutant allele has no visible phenotype and is completely recessive for enzyme effects at all stages tested. Segmental duplication of this region, including the aldox-2+ allele, has no apparent effect on the visible phenotype or the enzymatic activity. The mutant aldox-2 allele has no effect on the developmental expression of two unrelated enzymes, 6-phosphogluconate dehydrogenase and NADP+-dependent isocitrate dehydrogenase. The effects of this locus on aldehyde oxidase, xanthine dehydrogenase, and pyridoxal oxidase suggest that this locus may code for a product involved in the synthesis of the molybdenum cofactor common to these enzymes.
黑腹果蝇中的aldox - 2基因座已被证明对三种钼酶有不同影响，即醛氧化酶、吡哆醛氧化酶和黄嘌呤脱氢酶。这些影响在蛹 - 成虫边界附近最为明显，此时正常生物体以活性形式积累大量这些酶。该基因座已通过遗传学方法更精确地定位到2 - 82.9±2.1，所有重组染色体对这三种酶的影响完全一致。细胞遗传学定位也已确定在52E和54E8之间，且很可能位于54B1 - 54E8区域内。aldox - 2突变等位基因没有可见表型，并且在所有测试阶段对酶的影响都是完全隐性的。该区域的片段重复，包括aldox - 2 +等位基因，对可见表型或酶活性没有明显影响。突变的aldox - 2等位基因对两种不相关的酶，即6 - 磷酸葡萄糖酸脱氢酶和NADP +依赖的异柠檬酸脱氢酶的发育表达没有影响。该基因座对醛氧化酶、黄嘌呤脱氢酶和吡哆醛氧化酶的影响表明，该基因座可能编码一种参与这些酶共同的钼辅因子合成的产物。
