Martins H S, Martins-Filho O A, Araujo M Ss, Martins N R, Lagares M A
Departamento de Clinica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Laboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou - Fiocruz, Belo Horizonte, MG, Brazil.
Cryo Letters. 2018 Sep/Oct;39(5):298-305.
Frozen equine semen has lower fertility compared to cooled semen. Due to the difficulty to obtain equine oocytes, a heterologous zona pellucida binding assay (ZBA) is an alternative method to predict the fertilizing capability of equine frozen sperm. The rate of capacitated and hyperactivated sperm according to their motility characteristics were analyzed with a Computer Assisted Sperm Analyzer. We believe this report describes for the first time the in vitro hyperactivation induction and the heterologous ZBA to predict the fertilizing ability of frozen equine sperm.
This work aimed at developing an assay to evaluate the fertilizing ability of frozen equine sperm using a bovine ZBA with the use of an in vitro capacitation and hyperactivation media with procaine and calcium ionophore A23187, respectively.
The sperm motility characteristics, intact and acrosome reacted sperm rates, and number of stallion sperm bound to the bovine ZP were calculated.
The procaine group showed a hyperactivation motility pattern, although it improved ZP sperm binding similarly to the capacitation group.
The capacitation medium improved the IVF capability of frozen equine sperm, allowing the highest possibility of sperm-oocyte interaction.
与冷藏精液相比，冷冻马精液的生育力较低。由于获取马卵母细胞存在困难，一种异源透明带结合试验（ZBA）是预测冷冻马精子受精能力的替代方法。使用计算机辅助精子分析仪分析了根据其运动特征的获能和超活化精子的比例。我们相信本报告首次描述了体外超活化诱导和异源ZBA来预测冷冻马精子的受精能力。
本研究旨在开发一种试验，分别使用含有普鲁卡因和钙离子载体A23187的体外获能和超活化培养基，通过牛ZBA来评估冷冻马精子的受精能力。
计算精子运动特征、完整和顶体反应精子比例以及与牛透明带结合的种马精子数量。
普鲁卡因组显示出超活化运动模式，尽管其改善透明带精子结合的情况与获能组相似。
获能培养基提高了冷冻马精子的体外受精能力，使精子与卵母细胞相互作用的可能性最高。
