Ziboh V A, Wong T, Wu M C, Yunis A A
Biochem Biophys Res Commun. 1986 Oct 30;140(2):515-22. doi: 10.1016/0006-291x(86)90762-x.
The incubation of undifferentiated promyelocytic HL-60 cells with DMSO resulted in the rapid transmethylation of phosphatidyl ethanolamine (PE) into phosphatidylcholine (PC) which was maximal at 60 secs. This rapid generation of PC was followed by a decrease of the methylated phospholipid and the release of arachidonic acid. Thus, the rapid DMSO-induced phospholipid methylation coupled with release of arachidonic acid (precursor for eicosanoids) prior to morphological evidence of cellular differentiation may represent early biochemical events which result in the generation of intracellular chemical signals which may program the promyelocytic cells into a differentiation mode.
将未分化的早幼粒细胞HL-60细胞与二甲基亚砜（DMSO）一起孵育，导致磷脂酰乙醇胺（PE）迅速转甲基化为磷脂酰胆碱（PC），在60秒时达到最大值。PC的这种快速生成之后是甲基化磷脂的减少和花生四烯酸的释放。因此，在细胞分化的形态学证据出现之前，DMSO诱导的磷脂快速甲基化以及花生四烯酸（类花生酸的前体）的释放可能代表早期生化事件，这些事件导致细胞内化学信号的产生，这些信号可能将早幼粒细胞编程为分化模式。
